Eskelinen S
Department of Physiology, University of Oulu, Finland.
Can J Physiol Pharmacol. 1988 Jul;66(7):880-3. doi: 10.1139/y88-143.
The kinetics of drug-membrane interactions of erythrocytes from neonates were compared with those from adults by monitoring the time course of the shape transformations and vesicle release caused by drugs, using a light microscope--video recording technique. Both crenation caused by lysophosphatidylcholine (LPC) and cupping caused by chlorpromazine (CPZ) took place more slowly in the neonatal cells than in the cells from adults. The equilibrium concentrations of LPC and CPZ in erythrocytes did not differ significantly between the neonates and adults, however. The slower responses of the neonatal erythrocytes can be explained by the presence of negatively charged phosphatidylethanolamine and phosphatidylserine in the outer layer of the erythrocyte membrane, which may reduce the rate of incorporation of amphipathic LPC and attract cationic CPZ to remain in the outer membrane layer, lowering the rate of inward bending of the membrane normally caused by CPZ.
通过使用光学显微镜 - 视频记录技术监测药物引起的红细胞形状转变和囊泡释放的时间进程，比较了新生儿红细胞与成人红细胞的药物 - 膜相互作用动力学。溶血磷脂酰胆碱（LPC）引起的皱缩和氯丙嗪（CPZ）引起的凹陷在新生儿细胞中比在成人细胞中发生得更慢。然而，新生儿和成人红细胞中LPC和CPZ的平衡浓度没有显著差异。新生儿红细胞反应较慢可以通过红细胞膜外层中带负电荷的磷脂酰乙醇胺和磷脂酰丝氨酸的存在来解释，这可能会降低两亲性LPC的掺入速率，并吸引阳离子CPZ留在外膜层，从而降低通常由CPZ引起的膜向内弯曲的速率。
