Zhao Xueliang, Wang Wenlong, Bateer Huhe
Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Feb 25;36(2):250-258. doi: 10.13345/j.cjb.190238.
The objective of this study was to explore the link between long non-coding RNAs (lncRNAs) and resistance mechanism of albendazole in Haemonchus contortus by analyzing the gene expression profile of lncRNA in sensitive and resistant strains. This study provided the base for the resistance mechanism of Haemonchus contortus. In this experiment, the cDNA sequencing libraries were collected constructed for the sensitive and resistant strains of Haemonchus contortus, and the sequencing was performed using Illumina HiSeq 4000 platform, and differentially expressed lncRNAs (DEIncRNAs) were screened. Then the cis-targeted and trans-targeted genes of DElncRNAs were predicted, and the GO enrichment, KEGG pathway enrichment analysis were also made. The results displayed that 6 377 and 6 356 candidate lncRNA transcripts of sensitive and resistive strains were respectively obtained, 168 DElncRNAs of which were selected. Compared with resistive strains, there were 92 up-regulated and 76 down-regulated lncRNAs in sensitive strains. Meanwhile, 416 candidate target genes of DElncRNAs were obtained. The analytical results indicated that these genes participated in 641 GO terms and 92 pathways. The pathways involved in drug resistance are drug metabolism-other enzymes, drug metabolism-cytochrome P450, metabolism of xenobiotics by cytochrome P450 and so on. In conclusion, these findings inferred that some lncRNA-mediated target genes were associated with the resistance of Haemonchus contortus, and lncRNA may play an important role in the resistance of Haemonchus contortus. This study explored the expression profile of lncRNA in the Haemonchus contortus of sensitive and resistant strains. It was found that DElncRNAs in the sensitive and resistant strains, which helped to find out the Haemonchus contortus resistance mechanism of albendazole and provided a scientific basis for exploring the mechanism of resistance to Haemonchus contortus.
本研究的目的是通过分析捻转血矛线虫敏感株和抗性株中长链非编码RNA（lncRNA）的基因表达谱，探索lncRNA与捻转血矛线虫对阿苯达唑抗性机制之间的联系。本研究为捻转血矛线虫的抗性机制提供了依据。在本实验中，构建了捻转血矛线虫敏感株和抗性株的cDNA测序文库，使用Illumina HiSeq 4000平台进行测序，并筛选出差异表达的lncRNA（DEIncRNA）。然后预测DEIncRNA的顺式作用靶基因和反式作用靶基因，并进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路富集分析。结果显示，分别获得了敏感株和抗性株的6377个和6356个候选lncRNA转录本，其中筛选出168个DEIncRNA。与抗性株相比，敏感株中有92个lncRNA上调，76个lncRNA下调。同时，获得了416个DEIncRNA的候选靶基因。分析结果表明，这些基因参与了641个GO词条和92条通路。涉及抗性的通路有药物代谢-其他酶、药物代谢-细胞色素P450、细胞色素P450对外源化合物的代谢等。总之，这些发现推断一些lncRNA介导的靶基因与捻转血矛线虫的抗性有关，lncRNA可能在捻转血矛线虫的抗性中发挥重要作用。本研究探索了敏感株和抗性株捻转血矛线虫中lncRNA的表达谱。发现了敏感株和抗性株中的DEIncRNA，这有助于找出捻转血矛线虫对阿苯达唑的抗性机制，并为探索捻转血矛线虫的抗性机制提供科学依据。
