School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, China.
J Environ Manage. 2020 May 1;261:110237. doi: 10.1016/j.jenvman.2020.110237. Epub 2020 Mar 2.
Biosystems used for volatile organic compound (VOC) control have slow re-acclimation after extended starvation. In this study, a gel-encapsuled microorganism biofilter (GEBF) for the treatment of VOCs was used for rapid recovery after starvation interruption. Another conventional perlite biofilter (BF) was used as a control. Results showed that GEBF and BF needed 3 and 6 days for fully recovery after short-term (6 days) starvation. For long-term (20 days) starvation, GEBF fully recovered the removal performance after 9 days, whereas BF recovered only 70% within the same period. Flow cytometry analysis indicated that GEBF presented better viability state of microbial population than that in BF under starvation. The average metabolic activity of microorganisms in GEBF remained a relatively high during and after starvation (0.0049 h). However, the average metabolic activity of microorganisms in BF decreased from 0.0042 h before starvation to 0.0033 h under starvation. Changes in the microbial community structure in GEBF and BF were investigated and compared by high-throughput sequencing and principal component analysis. Notably, the microbial community structure in the two biofilters showed different behavior. All these results demonstrated that the gel encapsulation of microorganisms is a promising strategy to resist starvation in biofiltration technologies.
用于挥发性有机化合物（VOC）控制的生物系统在长时间饥饿后恢复缓慢。在这项研究中，使用凝胶包封微生物生物滤池（GEBF）来快速恢复饥饿中断后的处理 VOC 的能力。另一个常规的珍珠岩生物滤池（BF）被用作对照。结果表明，GEBF 和 BF 在短期（6 天）饥饿后分别需要 3 天和 6 天才能完全恢复。对于长期（20 天）饥饿，GEBF 在 9 天内完全恢复了去除性能，而 BF 在同一时期仅恢复了 70%。流式细胞术分析表明，与 BF 相比，GEBF 中微生物种群的生存能力更好。在饥饿期间和之后，GEBF 中微生物的平均代谢活性保持相对较高（0.0049 h）。然而，BF 中微生物的平均代谢活性从饥饿前的 0.0042 h 下降到饥饿时的 0.0033 h。通过高通量测序和主成分分析研究和比较了 GEBF 和 BF 中微生物群落结构的变化。值得注意的是，两个生物滤池中的微生物群落结构表现出不同的行为。所有这些结果表明，微生物的凝胶包封是生物过滤技术中抵抗饥饿的一种有前途的策略。
