Laksono James P, Sumirtanurdin Riyadi, Dania Haafizah, Ramadhani Fitri N, Perwitasari Dyah A, Abdulah Rizky, Barliana Melisa I
Department Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia.
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia.
J Pharm Bioallied Sci. 2019 Dec;11(Suppl 4):S601-S604. doi: 10.4103/jpbs.JPBS_216_19. Epub 2019 Dec 30.
Polymorphism on tryptophan hydroxylase 2 () gene rs120074175 can cause the synthesis of neurotransmitter serotonin in the brain to reduce up to 80%. Reduced serotonin in the brain can cause dopamine release to occur continuously. Excess dopamine in the brain may cause positive symptom of schizophrenia.
The aim of this study was to investigate the genotype distribution of rs120074175 gene on patients with schizophrenia at Prof. Dr. Soerojo Magelang Psychiatric Hospital, Indonesia, and the relationship between the genetic polymorphism of the rs120074175 gene against risk factors of schizophrenia.
This was a cross-sectional study.
The method used was amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Whole blood from healthy subjects and patients with schizophrenia, Wizard genomic deoxyribonucleic acid (DNA) purification kit (Promega, Fitchburg, Wisconsin), PCR master mix (Promega), ARMS-PCR primers, ddHO, agarose (Thermo Scientific, Seoul, South Korea), Tris, Acetic Acid, EDTA (TAE) 1X, ethidium bromide, loading dye 6×, and DNA ladder (Thermo Scientific) were the materials used.
Hardy-Weinberg equilibrium and chi-square (χ) tests were used.
The results showed that both groups (healthy subjects and patients with schizophrenia) at the Prof. Dr. Soerojo Magelang Psychiatric Hospital have a wild-type GG genotype (100%) without anyone having a mutant A allele.
rs120074175 gene polymorphism was not associated with risk factors for schizophrenia.
色氨酸羟化酶2（）基因rs120074175上的多态性可导致大脑中神经递质血清素的合成减少多达80%。大脑中血清素减少会导致多巴胺持续释放。大脑中多巴胺过多可能导致精神分裂症的阳性症状。
本研究旨在调查印度尼西亚梭罗佐马格朗精神病医院精神分裂症患者rs120074175基因的基因型分布，以及rs120074175基因的基因多态性与精神分裂症危险因素之间的关系。
这是一项横断面研究。
使用的方法是扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）。使用的材料包括来自健康受试者和精神分裂症患者的全血、Wizard基因组脱氧核糖核酸（DNA）纯化试剂盒（Promega，威斯康星州菲奇堡）、PCR预混液（Promega）、ARMS-PCR引物、ddHO、琼脂糖（赛默飞世尔科技，韩国首尔）、Tris、乙酸、乙二胺四乙酸（TAE）1X、溴化乙锭、6×上样染料和DNA Ladder（赛默飞世尔科技）。
采用哈迪-温伯格平衡和卡方（χ）检验。
结果显示，梭罗佐马格朗精神病医院的两组（健康受试者和精神分裂症患者）均具有野生型GG基因型（100%），无人携带突变A等位基因。
rs120074175基因多态性与精神分裂症危险因素无关。
