Samayanpaulraj Vignesh, Sivaramapillai Muthukumar, Palani Sankara Naynar, Govindaraj Krishnaveni, Velu Vijay, Ramesh Uthandakalaipandian
Department of Molecular Biology School of Biological Sciences Madurai Kamaraj University India.
Food Sci Nutr. 2020 Jan 20;8(2):1272-1283. doi: 10.1002/fsn3.1416. eCollection 2020 Feb.
, an inhabitant in the aquatic ecosystem is considered as an important foodborne bacterial zoonotic pathogen in aquaculture. The present study aimed to identify virulent from naturally infected in river Cauvery and in vitro evaluation of shrimp chitosan. Rimler Shotts (RS) and blood agar medium identified the presence of pathogenic sp. from the infected . Ah17 was identified using 16S rRNA nucleotide sequence. Extracellular enzymes such as amylase, lipase, and protease were screened in Ah17. Antibiotic susceptibility tests showed Ah17 was highly resistant against -lactam, glycopeptide, macrolides, phosphonic, fucidin, and oxazolidinone classes of antibiotics. Virulent genes such as hemolysin ( and , heat-labile enterotoxin (), cytotonic heat-stable enterotoxin (), elastase (), and lipase () were identified. Growth and the viable cell population of virulent Ah17 were significantly reduced in a dose-dependent manner against shrimp chitosan (CHS) from (). Thus, the present study isolated virulent Ah17 from the environmental source and characterized in vitro with shrimp chitosan.
作为水生生态系统中的一种生物,[具体细菌名称]被认为是水产养殖中一种重要的食源性细菌性人畜共患病原体。本研究旨在从卡弗里河中自然感染的[具体样本]中鉴定出有毒力的[具体细菌名称],并对虾壳聚糖进行体外评估。里姆勒-肖茨(RS)培养基和血琼脂培养基从感染的[具体样本]中鉴定出致病性[具体细菌名称]。使用16S rRNA核苷酸序列鉴定出Ah17。在[具体细菌名称]Ah17中筛选了淀粉酶、脂肪酶和蛋白酶等细胞外酶。抗生素敏感性试验表明,[具体细菌名称]Ah17对β-内酰胺类、糖肽类、大环内酯类、膦酸类、夫西地酸和恶唑烷酮类抗生素具有高度抗性。鉴定出了溶血素([具体基因名称1]和[具体基因名称2])、不耐热肠毒素([具体基因名称3])、细胞紧张性耐热肠毒素([具体基因名称4])、弹性蛋白酶([具体基因名称5])和脂肪酶([具体基因名称6])等毒力基因。有毒力的[具体细菌名称]Ah17的生长和活菌数量在剂量依赖性方式下显著降低,相对于来自[具体浓度范围]的虾壳聚糖(CHS)。因此,本研究从环境源中分离出有毒力的[具体细菌名称]Ah17,并在体外对其与虾壳聚糖进行了特性研究。