Antibacterial activity in secondary metabolite extracts of heterotrophic bacteria against and .

Disease causing bacteria such as and present a problem for fish farming. Treatment to remove them are generally carried out using antibiotics which have side effects on fish, the environment and humans. However, the use of antibacterial compounds derived from heterotrophic bacteria serve as a good alternative for antibiotics. Therefore, this study aimed to explore antibacterial activity in the secondary metabolite extracts of heterotrophic bacteria against and . : Heterotrophic bacteria namely JS04 MT102913.1, JS08 MT102920.1, JS10 MT102922.1, sp. JS11 MT102923.1, sp. JS19 MT102924.1, JS22 MT102926.1, and sp. strain JS25 MT102927.1 were used in this study. The sequences of these bacteria have been deposited and are available from NCBI GenBank. Each heterotrophic bacterium was cultured on 6L nutrient broth for 8 days, and extracts produced using ethyl acetate to obtain their secondary metabolites. These extracts were tested for their phytochemical contents using FT-IR and also tested for their inhibitory property in pathogenic bacteria by agar diffusion method. Phytochemical test results showed that the seven heterotrophic bacterial isolates produced terpenoid compounds. Based on the inhibitory test, the secondary metabolite extracts from sp strain JS04 had the highest inhibitory effect on the growth of pathogenic bacteria namely, (17.5 mm), (16.8 mm), and (17.3 mm). : It was concluded that the secondary metabolite extracts of heterotrophic bacteria inhibit the growth of and .