Alnimr Amani, Alamri Aisha, Alsultan Afnan
College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
College of Applied Medical Sciences Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Crit Care Res Pract. 2020 Feb 21;2020:3290316. doi: 10.1155/2020/3290316. eCollection 2020.
. Imipenem-resistant (IRAB) represents a major clinical threat. Dissemination in critical care areas necessitates effective action measures including genotyping tools to study the clonality of these strains and trace their origin. The main aim of this study is to assess the genetic relatedness between IRAB isolates in our institution intensive care units (ICU) which are at a particular risk of outbreaks.
Nonreplicate IRAB strains were serially collected over 3 years period (January 2016-December 2018) from patients admitted to the ICU. The isolates were phenotypically identified by a matrix-assisted laser desorption/ionization time-of-flight- (MALDI-TOF-) based system (VITEK MS), and their susceptibility was tested by the phenotypic-based VITEK 2 system. Molecular fingerprinting was performed by enterobacterial repetitive intergenic consensus (ERIC-PCR) followed by hierarchal clustering. The patterns were analysed by the software of BioNumerics package version 7.6.3 (Applied Maths, Belgium).
A total of eighty IRAB were isolated from 31 colonization and 59 infection sites in patients admitted to the ICU. Sixty-two samples were respiratory in origin (77.5%). The generated dendrogram revealed distinct patterns for majority (95%) of the strains. Meropenem maintained activity against 43.8% of the imipenem-resistant .
Meropenem can be a therapeutic option for imipenem-resistant .
耐亚胺培南鲍曼不动杆菌（IRAB）是一个主要的临床威胁。在重症监护区域的传播需要采取有效的行动措施，包括使用基因分型工具来研究这些菌株的克隆性并追踪其来源。本研究的主要目的是评估我们机构重症监护病房（ICU）中IRAB分离株之间的遗传相关性，这些病房存在爆发疫情的特殊风险。
在3年期间（2016年1月至2018年12月）从入住ICU的患者中连续收集非重复的IRAB菌株。通过基于基质辅助激光解吸/电离飞行时间（MALDI-TOF）的系统（VITEK MS）对分离株进行表型鉴定，并通过基于表型的VITEK 2系统测试其敏感性。通过肠杆菌重复基因间共识（ERIC-PCR）进行分子指纹图谱分析，然后进行层次聚类。通过BioNumerics软件包版本7.6.3（比利时应用数学公司）的软件分析图谱。
从入住ICU患者的31个定植部位和59个感染部位共分离出80株IRAB。62个样本来自呼吸道（77.5%）。生成的树状图显示大多数（95%）菌株有不同的图谱。美罗培南对43.8%的耐亚胺培南菌株保持活性。
美罗培南可作为耐亚胺培南菌株的一种治疗选择。
