Alamri Aisha M, Alsultan Afnan A, Ansari Mohammad A, Alnimr Amani M
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 34212, Saudi Arabia.
Department of Epidemic Disease Research, Institute of Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
Pathogens. 2020 Aug 2;9(8):630. doi: 10.3390/pathogens9080630.
This study analyzed the genotype, antibiotic resistance, and biofilm formation of strains and assessed the correlation between biofilm formation, antibiotic resistance, and biofilm-related risk factors. A total of 207 non-replicate multi-drug-resistant strains were prospectively isolated. Phenotypic identification and antimicrobial susceptibility testing were carried out. Isolate biofilm formation ability was evaluated using the tissue culture plate (TCP), Congo red agar, and tube methods. Clonal relatedness between the strains was assessed by enterobacterial repetitive intergenic consensus-PCR genotyping. Of the 207 isolates, 52.5% originated from an intensive care unit setting, and pan resistance was observed against ceftazidime and cefepime, with elevated resistance (99-94%) to piperacillin/tazobactam, imipenem, levofloxacin, and ciprofloxacin. alongside high susceptibility to tigecycline (97.8%). The Tissue culture plate, Tube method, and Congo red agar methods revealed that 53.6%, 20.8%, and 2.7% of the strains were strong biofilm producers, respectively, while a significant correlation was observed between biofilm formation and device-originating respiratory isolates ( = 0.0009) and between biofilm formation in colonized vs. true infection isolates ( = 0.0001). No correlation was detected between antibiotic resistance and biofilm formation capacity, and the majority of isolates were clonally unrelated. These findings highlight the urgent need for implementing strict infection control measures in clinical settings.
本研究分析了菌株的基因型、抗生素耐药性和生物膜形成情况，并评估了生物膜形成、抗生素耐药性与生物膜相关风险因素之间的相关性。前瞻性分离出总共207株非重复的多重耐药菌株。进行了表型鉴定和抗菌药敏试验。使用组织培养板（TCP）、刚果红琼脂和试管法评估分离株的生物膜形成能力。通过肠杆菌基因间重复一致序列-PCR基因分型评估菌株之间的克隆相关性。在207株分离株中，52.5%源自重症监护病房环境，观察到对头孢他啶和头孢吡肟的泛耐药，对哌拉西林/他唑巴坦、亚胺培南、左氧氟沙星和环丙沙星的耐药率升高（99%-94%）。同时对替加环素高度敏感（97.8%）。组织培养板法、试管法和刚果红琼脂法显示，分别有53.6%、20.8%和2.7%的菌株是强生物膜产生菌，而生物膜形成与器械来源的呼吸道分离株之间（P = 0.0009）以及定植与真正感染分离株中的生物膜形成之间（P = 0.0001）观察到显著相关性。未检测到抗生素耐药性与生物膜形成能力之间的相关性，并且大多数分离株在克隆上不相关。这些发现凸显了在临床环境中实施严格感染控制措施的迫切需求。
