Identification of key genes and pathways in gastric signet ring cell carcinoma based on transcriptome analysis.

BACKGROUND

Gastric signet ring cell carcinoma (GSRCC) is one of the most malignant tumors. It has the features of high invasiveness, rapid progression, and resistance to chemotherapy. However, systematic analyses of mRNAs have not yet been performed for GSRCC.

AIM

To identify key mRNAs and signaling pathways in GSRCC.

METHODS

A transcriptome analysis of two GSRCC and two non-GSRCC samples was performed in this study. Differentially expressed mRNAs and pathways were identified based on the KEGG and PANTHER pathway annotations. The interactive relationships among the differential genes were mapped with the STRING database. Quantitative real-time polymerase chain reaction was used to validate the key gene expression in GSRCC.

RESULTS

About 1162 differential genes (using a 2-fold cutoff, < 0.05) were identified in GSRCC compared with non-GSRCC. The enriched KEGG and PANTHER pathways for the differential genes included immune response pathways, metabolic pathways, and metastasis-associated pathways. Ten genes (, , , , , , , , , and ) were identified as hub genes in the protein-protein interaction network. The expression levels of five genes (, , , , and ) showed potential clinical value.

CONCLUSION

We have identified the potential key genes and pathways in GSRCC, and these hub genes and pathways could be diagnostic markers and therapeutic targets for GSRCC.