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人参皂苷和硒作为疫苗稀释剂的解决方案可提高小鼠的 Th1/Th2 免疫应答。

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice.

机构信息

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, 866 Yu Hang Tang Rd, Hangzhou, Zhejiang 310058, China.

出版信息

J Immunol Res. 2020 Feb 21;2020:2714257. doi: 10.1155/2020/2714257. eCollection 2020.

DOI:10.1155/2020/2714257
PMID:32149156
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7054799/
Abstract

Pseudorabies is an important infectious disease of swine, and immunization using attenuated pseudorabies virus (aPrV) vaccine is a routine practice to control this disease in swine herds. This study was to evaluate a saline solution containing ginseng stem-leaf saponins (GSLS) and sodium selenite (Se) as a vaccine adjuvant for its enhancement of immune response to aPrV vaccine. The results showed that aPrV vaccine diluted with saline containing GSLS-Se (aP-GSe) induced significantly higher immune responses than that of the vaccine diluted with saline alone (aP-S). The aP-GSe promoted higher production of gB-specific IgG, IgG1, and IgG2a, neutralizing antibody titers, secretion of Th1-type (IFN-, IL-2, IL-12), and Th2-type (IL-4, IL-6, IL-10) cytokines, and upregulated the T-bet/GATA-3 mRNA expression when compared to aP-S. In addition, cytolytic activity of NK cells, lymphocyte proliferation, and CD4/CD8 ratio was also significantly increased by aP-GSe. More importantly, aP-GSe conferred a much higher resistance of mice to a field virulent pseudorabies virus (fPrV) challenge. As the present study was conducted in mice, further study is required to evaluate the aP-GSe to improve the vaccination against PrV in swine.

摘要

伪狂犬病是一种重要的猪传染病，使用减毒伪狂犬病病毒（aPrV）疫苗进行免疫接种是控制猪群中这种疾病的常规做法。本研究旨在评估含有人参茎叶皂甙（GSLS）和亚硒酸钠（Se）的生理盐水作为疫苗佐剂对增强 aPrV 疫苗免疫反应的效果。结果表明，与单独用生理盐水稀释的疫苗（aP-S）相比，用含 GSLS-Se 的生理盐水稀释的 aPrV 疫苗（aP-GSe）诱导的免疫反应显著增强。与 aP-S 相比，aP-GSe 促进了更高水平的 gB 特异性 IgG、IgG1 和 IgG2a 的产生、中和抗体滴度、Th1 型（IFN-、IL-2、IL-12）和 Th2 型（IL-4、IL-6、IL-10）细胞因子的分泌，并上调了 T-bet/GATA-3 mRNA 的表达。此外，aP-GSe 还显著增加了 NK 细胞的细胞溶解活性、淋巴细胞增殖和 CD4/CD8 比值。更重要的是，aP-GSe 使小鼠对田间强毒伪狂犬病病毒（fPrV）的攻击具有更高的抵抗力。由于本研究是在小鼠中进行的，因此需要进一步研究 aP-GSe 以提高对猪伪狂犬病的疫苗接种效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff80/7054799/8b459559e424/JIR2020-2714257.008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff80/7054799/8b459559e424/JIR2020-2714257.008.jpg

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