Panghal Archna, Sathua Kshirod Bihari, Flora S J S
National Institute of Pharmaceutical Education and Research-Raebareli, NIPER Raebareli Transit Campus, Near CRPF Camp, Bijnor-Sisendi Road, P.O. Mati, Lucknow, 226002, Uttar Pradesh, India.
Heliyon. 2020 Feb 19;6(2):e03431. doi: 10.1016/j.heliyon.2020.e03431. eCollection 2020 Feb.
Arsenic (As) is naturally occurring toxic metalloid which is considered as a serious environmental and health concern. Red blood cells are the prime target for any toxicants as their population is higher in systemic circulation. High prevalence of anaemia too has been reported from arsenic contaminated area, suggesting possible linkage between arsenic and the damaging effects on RBCs. The exact mechanism for these effects is still not clear, however, oxidative/nitrosative stress might be one of the causative factors to play a key role. The present study was planned to evaluate the protective effects of a metal chelator, MiADMSA either alone or in combination with a natural antioxidant (gallic acid) for the reversal of arsenic induced oxidative damage in red blood cells. We collected rat RBCs and cultured them in appropriate medium. They were incubated with MiADMSA and gallic acid and then treated with sodium arsenite at 37 °C. Hemolysates were prepared and assayed for various biochemical parameters such as oxidative/nitrosative variables, osmotic fragility, acetylcholinesterase activity, and cellular metal accumulation. We found there was reversibility of oxidative/nitrosative stress variables, elevated cellular antioxidant power, and decreased osmotic fragility of red blood cells both in MiADMSA alone as well as in combination with gallic acid treated group compared with arsenic treated group. In conclusion, MiADMSA efficiently participated in the reversal of arsenic induced oxidative/nitrosative damage in red blood cells where as Gallic acid improved its reversal when given in combination with MiADMSA.
砷（As）是一种天然存在的有毒类金属，被视为严重的环境和健康问题。红细胞是任何有毒物质的主要靶标，因为它们在体循环中的数量较多。砷污染地区也报告了贫血的高患病率，这表明砷与对红细胞的损害作用之间可能存在联系。然而，这些影响的确切机制仍不清楚，氧化/亚硝化应激可能是起关键作用的致病因素之一。本研究旨在评估金属螯合剂二巯基丁二酸单酰胺（MiADMSA）单独或与天然抗氧化剂（没食子酸）联合使用对逆转砷诱导的红细胞氧化损伤的保护作用。我们收集大鼠红细胞并在适当的培养基中培养。将它们与MiADMSA和没食子酸一起孵育，然后在37℃下用亚砷酸钠处理。制备溶血产物并测定各种生化参数，如氧化/亚硝化变量、渗透脆性、乙酰胆碱酯酶活性和细胞金属积累。我们发现，与砷处理组相比，单独使用MiADMSA以及与没食子酸联合处理组的红细胞氧化/亚硝化应激变量具有可逆性，细胞抗氧化能力增强，渗透脆性降低。总之，MiADMSA有效地参与了逆转砷诱导的红细胞氧化/亚硝化损伤，而没食子酸与MiADMSA联合使用时可改善其逆转效果。
