锥形双模式光纤中接近色散转折点处IgG的高灵敏度检测

High-Sensitivity Detection of IgG Operating near the Dispersion Turning Point in Tapered Two-Mode Fibers.

作者信息

Sun Bing, Wang Yiping

机构信息

Guangdong and Hong Kong Joint Research Centre for Optical Fibre Sensors, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.

出版信息

Micromachines (Basel). 2020 Mar 5;11(3):270. doi: 10.3390/mi11030270.

DOI:10.3390/mi11030270

PMID:32150814

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7142735/

Abstract

The conventional methods for monitoring IgG levels suffer from some apparent problems such as long assay time, multistep processing, and high overall cost. An effective and suitable optical platform for label-free biosensing was investigated by the implementation of antibody/antigen immunoassays. The ultrasensitive detection of IgG levels could be achieved by exploiting the dispersion turning point (DTP) existing in the tapered two-mode fibers (TTMFs) because the sensitivity will reach ±∞ on either side of the DTP. Tracking the resonant wavelength shift, it was found that the fabricated TTMF device exhibited limits of detection (LOD) down to concentrations of 10 fg/mL of IgG in PBS solution. Such immunosensors based on DTP have great significance on trace detection of IgG due to simple detection scheme, quick response time, and miniaturization.

摘要

传统的监测IgG水平的方法存在一些明显的问题，如检测时间长、多步骤处理和总体成本高。通过实施抗体/抗原免疫测定法，研究了一种有效且合适的用于无标记生物传感的光学平台。利用锥形双模式光纤（TTMFs）中存在的色散转折点（DTP），可以实现IgG水平的超灵敏检测，因为在DTP的两侧灵敏度将达到±∞。通过跟踪共振波长的移动，发现所制备的TTMF器件在PBS溶液中对IgG的检测限低至10 fg/mL的浓度。这种基于DTP的免疫传感器由于检测方案简单、响应时间快和小型化，在IgG的痕量检测方面具有重要意义。