基于过度倾斜光纤光栅的人体心力衰竭生物标志物免疫传感器。
Human heart failure biomarker immunosensor based on excessively tilted fiber gratings.
作者信息
Luo Binbin, Wu Shengxi, Zhang Zhonghao, Zou Wengen, Shi Shenghui, Zhao Mingfu, Zhong Nianbing, Liu Yong, Zou Xue, Wang Lingling, Chai Weina, Hu Chuanmin, Zhang Lin
机构信息
Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, 400054, China; School of Opto-electronic Information, University of Electronic Science and Technology of China, Chengdu, 610054, China; Aston Institute of Photonic Technologies, Aston University, Birmingham, B4 7ET, UK;
School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.
出版信息
Biomed Opt Express. 2016 Dec 5;8(1):57-67. doi: 10.1364/BOE.8.000057. eCollection 2017 Jan 1.
A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of 0.5ng/mL for NT-proBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 01.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
基于过度倾斜光纤光栅（Ex-TFGs）开发了一种无标记免疫传感器平台，用于高特异性和快速检测人N端前B型利钠肽（NT-proBNP），NT-proBNP被认为是心力衰竭（HF）预后和风险分层的有力生物标志物。通过葡萄球菌蛋白A（SPA）法将本实验室制备的高纯度抗NT-proBNP单克隆抗体（MAbs）固定在光纤表面，用于后续靶向NT-proBNP的特异性结合。利用光纤光栅解调系统（FOGDS），通过监测固定有抗NT-proBNP MAbs的Ex-TFG生物传感器的共振波长偏移进行免疫测定。获得了NT-proBNP的最低可检测浓度约为0.5ng/mL，在0~1.0 ng/mL浓度范围内对NT-proBNP的平均灵敏度约为45.967 pm/（ng/mL）。所提出的Ex-TFG生物标志物传感器对几种人血清样本进行了评估，对NT-proBNP具有高特异性，表明其在急性HF症状患者早期诊断中的潜在应用。
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