基于衰减全反射表面增强红外吸收光谱（ATR-SEIRAS）的金表面上 EDC/NHS 固定化的原位研究。

In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS).

机构信息

Department of Biomedical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan.

Department of Biomedical Engineering, National Cheng Kung University, Tainan, 70101, Taiwan; Department of Engineering Science, National Cheng Kung University, Tainan, 70101, Taiwan.

出版信息

Colloids Surf B Biointerfaces. 2019 Mar 1;175:300-305. doi: 10.1016/j.colsurfb.2018.12.009. Epub 2018 Dec 6.

DOI:10.1016/j.colsurfb.2018.12.009

PMID:30553205

Abstract

In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

摘要

在这项研究中，使用表面增强红外吸收光谱（SEIRAS）精确地描述了 11-巯基十一酸（11-MUA）与 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）之间的时间依赖性反应。根据 SEIRAS 的光谱结果与电化学行为之间的高度相关性，强烈表明在中性 pH 条件（pH = 7.0）下，磷酸盐离子会明显干扰 EDC/NHS 反应。