Graduate Department of Life and Pharmaceutical Sciences and the Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul 03760, Korea.
Department of Life Science, Sogang University, Seoul 04107, Korea.
Int J Mol Sci. 2020 Mar 5;21(5):1787. doi: 10.3390/ijms21051787.
Low-oxygen stress, mainly caused by soil flooding, is a serious abiotic stress affecting crop productivity worldwide. To understand the mechanisms of low-oxygen stress responses and adaptation of plants, we characterized and compared low-oxygen responses in six species with different accessions of the Brassicaceae family. Based on the growth and survival responses to submergence or low-oxygen condition, these accessions could be divided into three groups: (i) Highly tolerant species ( and ); (ii) moderately tolerant species ( [, Ler, Ws and Col-0 ecotype]); and (iii) intolerant species (, [Shandong and Yukon ecotype], and ). Gene expression profiling using Operon Arabidopsis microarray was carried out with RNA from roots of (Col-0), , , and (Shandong) treated with low-oxygen stress (0.1% O/99.9% N) for 0, 1, 3, 8, 24, and 72 h. We performed a comparative analysis of the gene expression profiles using the gene set enrichment analysis (GSEA) method. Our comparative analysis suggested that under low-oxygen stress each species distinctively reconfigures the energy metabolic pathways including sucrose-starch metabolism, glycolysis, fermentation and nitrogen metabolism, tricarboxylic acid flow, and fatty acid degradation via beta oxidation and glyoxylate cycle. In , a moderately tolerant species, the dynamical reconfiguration of energy metabolisms occurred in the early time points of low-oxygen treatment, but the energy reconfiguration in the late time points was not as dynamic as in the early time points. Highly tolerant appeared to have high photosynthesis capacity that could produce more O and in turn additional ATP energy to cope with energy depletion caused by low-oxygen stress. seemed to retain some ATP energy produced by anaerobic energy metabolism during a prolonged period of low-oxygen conditions. Intolerant did not show significant changes in the expression of genes involved in anaerobic energy metabolisms. These results indicate that plants developed different energy metabolisms to cope with the energy crisis caused by low-oxygen stress.
低氧胁迫主要由土壤淹水引起，是一种严重的非生物胁迫，影响全球作物生产力。为了了解植物对低氧胁迫的响应和适应机制，我们对来自 6 个不同芸薹科物种的低氧响应进行了特征描述和比较。根据对淹没或低氧条件下的生长和存活响应，这些品系可分为 3 组：（i）高耐受物种（和）；（ii）中耐受物种（[， Ler，Ws 和 Col-0 生态型]）；和（iii）不耐受物种（，[山东和育空生态型]和）。使用 Operon Arabidopsis 微阵列对低氧胁迫（0.1% O/99.9% N）处理 0、1、3、8、24 和 72 h 后的根中的 RNA 进行基因表达谱分析，供试材料为（Col-0）、、、和（山东）。我们使用基因集富集分析（GSEA）方法对基因表达谱进行了比较分析。我们的比较分析表明，在低氧胁迫下，每个物种都通过蔗糖-淀粉代谢、糖酵解、发酵和氮代谢、三羧酸循环以及脂肪酸降解通过β氧化和乙醛酸循环，来显著重新配置能量代谢途径。在中耐受物种中，能量代谢的动态重配置发生在低氧处理的早期时间点，但在晚期时间点的能量重配置不如早期时间点动态。高耐受物种似乎具有较高的光合作用能力，可以产生更多的 O 和相应的 ATP 能量，以应对低氧胁迫引起的能量耗竭。似乎在长时间的低氧条件下保留了一些由厌氧能量代谢产生的 ATP 能量。不耐受物种没有表现出参与厌氧能量代谢的基因表达的显著变化。这些结果表明，植物发展了不同的能量代谢来应对低氧胁迫引起的能量危机。
Zotero 插件
