De Bellis Luigi, Luvisi Andrea, Alpi Amedeo
Department of Biological and Environmental Sciences and Technologies, University of Salento, Via Prov. le Monteroni, I-73100 Lecce, Italy.
Approaching Research Educational Activities (A.R.E.A.) Foundation, I-56126 Pisa, Italy.
Biology (Basel). 2020 Jul 12;9(7):162. doi: 10.3390/biology9070162.
After the discovery in 1967 of plant glyoxysomes, aconitase, one the five enzymes involved in the glyoxylate cycle, was thought to be present in the organelles, and although this was found not to be the case around 25 years ago, it is still suggested in some textbooks and recent scientific articles. Genetic research (including the study of mutants and transcriptomic analysis) is becoming increasingly important in plant biology, so metabolic pathways must be presented correctly to avoid misinterpretation and the dissemination of bad science. The focus of our study is therefore aconitase, from its first localization inside the glyoxysomes to its relocation. We also examine data concerning the role of the enzyme malate dehydrogenase in the glyoxylate cycle and data of the expression of aconitase genes in Arabidopsis and other selected higher plants. We then propose a new model concerning the interaction between glyoxysomes, mitochondria and cytosol in cotyledons or endosperm during the germination of oil-rich seeds.
1967年发现植物乙醛酸循环体后，乌头酸酶（参与乙醛酸循环的五种酶之一）被认为存在于这些细胞器中，尽管大约25年前发现并非如此，但在一些教科书和近期的科学文章中仍有此说法。在植物生物学中，遗传学研究（包括突变体研究和转录组分析）正变得越来越重要，因此必须正确呈现代谢途径，以避免误解和不良科学的传播。因此，我们研究的重点是乌头酸酶，从其最初在乙醛酸循环体中的定位到重新定位。我们还研究了有关苹果酸脱氢酶在乙醛酸循环中作用的数据以及拟南芥和其他选定高等植物中乌头酸酶基因的表达数据。然后，我们提出了一个关于富含油种子萌发过程中子叶或胚乳中乙醛酸循环体、线粒体和细胞质之间相互作用的新模型。
