Department of Microbiology, Faculty of Biological Science, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Department of Influenza and Other Respiratory Viruses, Pasteur Institute of Iran, 69, 1316943551, Tehran, Iran.
Biotechnol Lett. 2020 Jul;42(7):1147-1159. doi: 10.1007/s10529-020-02822-3. Epub 2020 Mar 9.
Vaccination is the most effective preventive strategy for influenza disease. As the virus undergoes high antigenic drift, it requires a constant reformulation to obtain high protection.
Immunogenicity of a purified chimeric protein containing conserved regions of influenza A/H1N1 viruses including the Hemagglutinin stalk domain, Nucleoprotein, and Matrix protein produced in a prokaryotic system was assessed in vitro and in vivo, alone or in combination with adjuvants by evaluating antibody responses, cytokine production, lymphocyte proliferative assay, and mortality rate after challenge. The animals that received the chimeric protein had specific antibody responses, elicited memory CD4 cells, cytokines of Th1 and Th2 cells and showed 75% protection against influenza virus lethal challenge. The animals injected with the chimeric protein supplemented with Alum showed improved immune responses, but they had 67% protection. In other words, although Alum adjuvant enriched the chimera specific immune responses potently, it could not enhance its protectivity.
Regarding the immunogenicity and protectivity of the chimeric protein construct against influenza, findings of the study suggested that the chimeric protein could be considered as a promising influenza vaccine candidate.
接种疫苗是预防流感疾病最有效的策略。由于病毒具有高度的抗原漂移性，因此需要不断进行配方改革以获得高度保护。
在体外和体内评估了在原核系统中生产的包含流感 A/H1N1 病毒保守区域的纯化嵌合蛋白（包括血凝素茎结构域、核蛋白和基质蛋白）的免疫原性，单独或与佐剂联合使用，通过评估抗体反应、细胞因子产生、淋巴细胞增殖试验和攻毒后的死亡率。接受嵌合蛋白的动物具有特异性抗体反应，引发记忆性 CD4 细胞、Th1 和 Th2 细胞的细胞因子，并对流感病毒致死性攻毒具有 75%的保护率。用 Alum 佐剂补充的嵌合蛋白注射的动物显示出改善的免疫反应，但保护率为 67%。换句话说，尽管 Alum 佐剂强烈增强了嵌合蛋白的特异性免疫反应，但不能增强其保护力。
关于该嵌合蛋白构建体针对流感的免疫原性和保护力，研究结果表明，该嵌合蛋白可以被视为有前途的流感疫苗候选物。
