The USF Health Heart Institute, University of South Florida, Tampa, FL, USA.
College of Pharmacy, University of South Florida, Tampa, FL, USA.
Methods Mol Biol. 2020;2118:111-120. doi: 10.1007/978-1-0716-0319-2_8.
Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and D-phenylalanyl-L-prolyl-L-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.
凝血酶是凝血级联反应中涉及的主要蛋白质，可将无活性的纤维蛋白原转化为纤维蛋白，在血栓形成的发展中起着至关重要的作用。抗凝血酶纳米颗粒能够实现靶向抗凝而不增加出血风险。本文概述了制备和鉴定水蛭素和 D-苯丙氨酰-L-脯氨酰-L-精氨酰-氯甲基酮（PPACK）纳米颗粒的过程。此外，还描述了这些纳米颗粒的特性，包括粒径、zeta 电位和 PPACK/水蛭素载药量的定量。
