Núcleo Multidisciplinar de Pesquisa em Biologia-Duque de Caxias, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Methods Mol Biol. 2020;2118:235-250. doi: 10.1007/978-1-0716-0319-2_18.
Mesenchymal stem cell (MSC) therapy has emerged as a potential therapeutic option for several diseases due to their unique properties of releasing important bioactive factors. Despite the advances in stem cell therapy, it is still difficult to accurately determine the mechanisms of cell activities after in vivo transplantation. The application of noninvasive cell tracking approaches is important to determine tissue distribution and the lifetime of stem cells following their injection, which consequently provides knowledge about the mechanisms of stem cell tissue repair. Superparamagnetic iron oxide nanoparticles (SPION) can provide a very useful tool for labeling and tracking stem cells by magnetic resonance imaging without causing toxic cellular effects and do not elicit any other side effects. Here we describe how to use SPIONs to label mesenchymal stem cells and evaluate efficacy and potential cytotoxicity in vitro.
间充质干细胞（MSC）疗法因其释放重要生物活性因子的独特特性，已成为多种疾病的潜在治疗选择。尽管干细胞治疗取得了进展，但仍难以准确确定体内移植后细胞活性的机制。应用非侵入性细胞跟踪方法对于确定干细胞注射后的组织分布和寿命非常重要，这反过来又提供了关于干细胞组织修复机制的知识。超顺磁氧化铁纳米粒子（SPION）可以通过磁共振成像为标记和跟踪干细胞提供非常有用的工具，而不会造成细胞毒性，也不会引起任何其他副作用。在这里，我们描述了如何使用 SPION 标记间充质干细胞，并评估体外的功效和潜在细胞毒性。
