Eser Muhammed Taha, Bektasoglu Pinar Kuru, Gurer Bora, Bozkurt Huseyin, Sorar Mehmet, Ozturk Ozden Caglar, Arikok Ata Turker, Kertmen Hayri
Turkish Ministry of Health, University of Health Sciences, Diskapı Education and Research Hospital, Department of Neurosurgery, Ankara, Turkey.
Turk Neurosurg. 2020;30(2):163-170. doi: 10.5137/1019-5149.JTN.24647-18.3.
To investigate the effects of an anti-ischemic agent, mildronate, on subarachnoid hemorrhage-induced vasospasm.
Rabbits were randomly divided into four groups: control, subarachnoid hemorrhage (SAH), vehicle, and mildronate (n=8 animals per group). In the treatment group, 200 mg/kg of mildronate were intraperitoneally administered 5 minutes after the procedure and continued for 3 days as daily administrations of the same dose. At the end of the third day, the cerebrum, cerebellum, and brain stem were perfused, fixated, and removed for histopathological examination. Tissues were examined for arterial wall thickness, luminal area, and hippocampal neuronal degeneration.
Mildronate group showed significantly increased luminal area and reduced wall thickness of the basilar artery compared with the subarachnoid hemorrhage group. In addition, the hippocampal cell degeneration score was significantly lower in the mildronate group than in the SAH and vehicle groups.
These results show that mildronate exerts protective effects against SAH-induced vasospasm and secondary neural injury.
研究抗缺血药物米屈膦酸盐对蛛网膜下腔出血所致血管痉挛的影响。
将家兔随机分为四组：对照组、蛛网膜下腔出血（SAH）组、赋形剂组和米屈膦酸盐组（每组8只动物）。在治疗组中，术后5分钟腹腔注射200mg/kg米屈膦酸盐，并以相同剂量每日给药，持续3天。在第三天结束时，对大脑、小脑和脑干进行灌注、固定并取出进行组织病理学检查。检查组织的动脉壁厚度、管腔面积和海马神经元变性情况。
与蛛网膜下腔出血组相比，米屈膦酸盐组基底动脉的管腔面积显著增加，管壁厚度减小。此外，米屈膦酸盐组的海马细胞变性评分显著低于SAH组和赋形剂组。
这些结果表明，米屈膦酸盐对SAH诱导的血管痉挛和继发性神经损伤具有保护作用。
