College of Chemistry & Environmental Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding 071002, P. R. China.
Medical College, Hebei University, Baoding 071002, P. R. China.
ACS Appl Mater Interfaces. 2020 Apr 1;12(13):14866-14875. doi: 10.1021/acsami.9b22979. Epub 2020 Mar 19.
Light-responsive nanoprobes were suffering from the threat of high-dose laser irradiation, and it was important for constructing new nanoprobes for safe and efficient phototheranostics. Here, polydopamine (PDA)-coated gold nanobipyramids (AuNBPs@PDA) were synthesized for amplified photoacoustic (PA) signal and enhanced photothermal conversion with low-dose laser irradiation and then doxorubicin (DOX)-loaded AuNBPs@PDA-DOX nanoprobes were constructed for PA imaging-guided synergistic photothermal therapy (PTT) and chemotherapy. The AuNBPs@PDA nanoparticles possessed higher photothermal conversion efficiency (42.07%) and stronger PA signal than those of AuNBP nanoparticles, and the AuNBPs@PDA-DOX nanoprobes showed dual-responsive DOX release of pH and photothermal stimulation. With low-dose laser irradiation (1.0 W/cm) and low-concentration AuNBPs@PDA-DOX (60 μg/mL), the 4T1 cell viability was reduced to about 5%, owing to the combination of PTT and chemotherapy, compared with 42.3% of single chemotherapy and 25.3% of single PTT. Moreover, by modeling 4T1 tumor-bearing nude mice, in vivo PA imaging was achieved and the tumors were completely inhibited, demonstrating the excellent synergistic effect of PTT/chemotherapy. Therefore, the developed AuNBPs@PDA-DOX nanoprobes can be used for phototheranostics and synergistic chemotherapy, achieving low-dose laser irradiation and high-efficient visualized theranostics.
光响应纳米探针受到高剂量激光照射的威胁，因此构建用于安全高效光治疗的新型纳米探针非常重要。在此，合成了聚多巴胺（PDA）包覆的金纳米双锥体（AuNBPs@PDA），用于低剂量激光照射下放大光声（PA）信号和增强光热转换，然后构建载多柔比星（DOX）的 AuNBPs@PDA-DOX 纳米探针，用于 PA 成像引导协同光热治疗（PTT）和化学疗法。AuNBPs@PDA 纳米粒子具有比 AuNBP 纳米粒子更高的光热转换效率（42.07%）和更强的 PA 信号，并且 AuNBPs@PDA-DOX 纳米探针表现出 pH 和光热刺激双重响应的 DOX 释放。在低剂量激光照射（1.0 W/cm）和低浓度 AuNBPs@PDA-DOX（60μg/mL）下，由于 PTT 和化学疗法的结合，4T1 细胞活力降低至约 5%，而单独化学疗法为 42.3%，单独 PTT 为 25.3%。此外，通过建模 4T1 荷瘤裸鼠，实现了体内 PA 成像，并且肿瘤完全被抑制，证明了 PTT/化学疗法的协同增效作用。因此，开发的 AuNBPs@PDA-DOX 纳米探针可用于光治疗和协同化学疗法，实现低剂量激光照射和高效可视化治疗。
