Chen Bo, Mei Lan, Fan Rangrang, Chuan Di, Ren Yangmei, Mu Min, Chen Haifeng, Zou Bingwen, Guo Gang
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Asian J Pharm Sci. 2023 Mar;18(2):100781. doi: 10.1016/j.ajps.2023.100781. Epub 2023 Feb 1.
The combination of photothermal therapy with chemotherapy has gradually developed into promising cancer therapy. Here, a synergistic photothermal-chemotherapy nanoplatform based on polydopamine (PDA)-coated gold nanoparticles (AuNPs) were facilely achieved the polymerization of dopamine (DA) on the surface of AuNPs. This nanoplatform exhibited augmented photothermal conversion efficiency and enhanced colloidal stability in comparison with uncoated PDA shell AuNPs. The i-motif DNA nanostructure was assembled on PDA-coated AuNPs, which could be transformed into a C-quadruplex structure under an acidic environment, showing a characteristic pH response. The PDA shell served as a linker between the AuNPs and the i-motif DNA nanostructure. To enhance the specific cellular uptake, the AS1411 aptamer was introduced to the DNA nanostructure employed as a targeting ligand. In addition, Dox-loaded NPs (DAu@PDA-AS141) showed the pH/photothermal-responsive release of Dox. The photothermal effect of DAu@PDA-AS141 elicited excellent photothermal performance and efficient cancer cell inhibition under 808 nm near-infrared (NIR) irradiation. Overall, these results demonstrate that the DAu@PDA-AS141 nanoplatform shows great potential in synergistic photothermal-chemotherapy.
光热疗法与化疗相结合已逐渐发展成为一种很有前景的癌症治疗方法。在此，基于聚多巴胺（PDA）包覆的金纳米颗粒（AuNPs）构建了一种协同光热化疗纳米平台，通过在AuNPs表面使多巴胺（DA）聚合轻松实现。与未包覆PDA壳的AuNPs相比，该纳米平台表现出更高的光热转换效率和更强的胶体稳定性。i-基序DNA纳米结构组装在PDA包覆的AuNPs上，在酸性环境下可转变为C-四链体结构，呈现出特征性的pH响应。PDA壳充当AuNPs与i-基序DNA纳米结构之间的连接体。为增强细胞特异性摄取，将AS1411适配体引入用作靶向配体的DNA纳米结构中。此外，载有多柔比星的纳米颗粒（DAu@PDA-AS141）显示出多柔比星的pH/光热响应释放。DAu@PDA-AS141的光热效应在808 nm近红外（NIR）照射下引发了优异的光热性能和高效的癌细胞抑制作用。总体而言，这些结果表明DAu@PDA-AS141纳米平台在协同光热化疗方面具有巨大潜力。
