Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Hum Exp Toxicol. 2020 Aug;39(8):1019-1030. doi: 10.1177/0960327120909525. Epub 2020 Mar 10.
Diabetes mellitus (DM) is a complex metabolic disease and it is also closely associated with a reduction in fertility in male patients. The purpose of the present study was to investigate the antidiabetic effect of (CRV), as a potent antioxidant, on the numbers of germ cells and Sertoli cells in testicular tissue, and the messenger RNA (mRNA) and protein expression of some genes involved in spermatogenesis, including luteinizing hormone/choriogonadotropin receptor (), follicle-stimulating hormone receptor (), and steroidogenic factor 1 (), as well as hormones such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), and insulin. Adult male Wistar rats ( = 32) were randomly divided into four groups (eight animals per group), including healthy control that received 0.2% Tween 80, diabetic control group, the diabetic group treated orally with CRV (75 mg/kg), and CRV group that received orally CRV (75 mg/kg). The duration of the treatment period lasted 8 weeks. In the diabetic group, the numbers of Sertoli cells and germ cells were significantly decreased, while the treatment with CRV prevented the degree of the damage to the cells mentioned earlier. CRV administration elevated the concentrations of insulin, T, FSH, and LH. Moreover, treatment with CRV significantly enhanced the levels of the mRNA and protein expression of , , and . According to the obtained results, CRV administration could prevent the deleterious effects of DM on testicular germ cells, and it increases the levels of hormones and some essential genes, such as , , and , involved in the process of spermatogenesis.
糖尿病（DM）是一种复杂的代谢性疾病，它与男性患者生育能力下降密切相关。本研究旨在探讨（CRV）作为一种有效的抗氧化剂，对睾丸组织中生殖细胞和支持细胞数量的影响，以及一些参与精子发生的基因的信使 RNA（mRNA）和蛋白表达，包括促黄体激素/绒毛膜促性腺激素受体（）、卵泡刺激素受体（）和类固醇生成因子 1（），以及黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）和胰岛素等激素。成年雄性 Wistar 大鼠（n = 32）随机分为四组（每组 8 只），包括健康对照组（给予 0.2%Tween 80）、糖尿病对照组、糖尿病组口服 CRV（75mg/kg）和 CRV 组（给予 75mg/kg）口服 CRV。治疗期持续 8 周。在糖尿病组中，支持细胞和生殖细胞的数量明显减少，而 CRV 治疗可预防上述细胞损伤的程度。CRV 给药可提高胰岛素、T、FSH 和 LH 的浓度。此外，CRV 治疗可显著增强、和的 mRNA 和蛋白表达水平。根据所得结果，CRV 给药可预防 DM 对睾丸生殖细胞的有害影响，并可增加激素和一些重要基因（如、和）的水平，这些基因参与精子发生过程。
