Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones, CONICET, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones (UNaM), Posadas, Misiones, Argentina.
Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA/CONICET), Los Reseros y Nicolas Repetto, Hurlingham, Buenos Aires, Argentina.
Prep Biochem Biotechnol. 2020;50(8):753-762. doi: 10.1080/10826068.2020.1734941. Epub 2020 Mar 10.
Agricultural practices generate lignocellulosic waste that can be bioconverted by fungi to generate value-added products such as biofuels. In this context, fungal enzymes are presented as an alternative for their use in the hydrolysis of cellulose to sugars that can be fermented to ethanol. The aim of this work was to characterize LBM 033 strain and to analyze its efficiency in the hydrolysis of cellulosic substrates, including barley straw. LBM 033 strain was identified as by molecular techniques, through the use of the ITS and rbp2 markers and the construction of phylogenetic trees. The cell-free supernatant of LBM 033 showed high titers of hydrolytic enzymatic activities, necessary for the hydrolysis of the holocellulosic substrates, hydrolyzing pure cellulose to cellobiose and glucose and also degraded the polysaccharides contained in barley straw to short soluble oligosaccharides. These results indicate that macro fungi from tropical soil environments, such as LBM 033 can be a valuable resource for in-house, cost effective production of enzymes that can be applied in the hydrolysis stage, which could reduce the total cost of bioethanol production.
农业生产会产生木质纤维素废料，这些废料可以被真菌生物转化为增值产品，如生物燃料。在这种情况下，真菌酶被认为是替代纤维素水解为可发酵成乙醇的糖的方法。本工作的目的是对 LBM 033 菌株进行表征，并分析其在纤维素底物水解中的效率，包括大麦秸秆。通过使用 ITS 和 rbp2 标记物以及构建系统发育树，通过分子技术将 LBM 033 菌株鉴定为 。LBM 033 的无细胞上清液显示出高水解酶活性，这对于水解全纤维素底物是必要的，它可以将纯纤维素水解为纤维二糖和葡萄糖，还可以将大麦秸秆中的多糖降解为短链可溶性寡糖。这些结果表明，来自热带土壤环境的大型真菌，如 LBM 033，可以成为内部、具有成本效益的酶生产的有价值资源，这些酶可以应用于水解阶段，从而降低生物乙醇生产的总成本。
