Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, Canada.
Bioresour Technol. 2011 Nov;102(21):10020-7. doi: 10.1016/j.biortech.2011.08.045. Epub 2011 Aug 17.
The use of Trametes versicolor as a biological pretreatment for canola straw was explored in the context of biofuel production. Specifically, the effects on the straw of a wild-type strain (52J) and a cellobiose dehydrogenase (CDH)-deficient strain (m4D) were investigated. The xylose and glucose contents of the straw treated with 52J were significantly reduced, while only the xylose content was reduced with m4D treatment. Lignin extractability was greatly improved with fungal treatments compared to untreated straw. Saccharification of the residue of the m4D-treated straw led to a significant increase in proportional glucose yield, which was partially attributed to the lack of cellulose catabolism by m4D. Overall, the results of this study indicate that CDH facilitates cellulose access by T. versicolor. Furthermore, treatment of lignocellulosic material with m4D offers improvements in lignin extractability and saccharification efficacy compared to untreated biomass without loss of substrate due to fungal catabolism.
以生物燃料生产为背景，研究了糙皮侧耳（Trametes versicolor）作为一种生物预处理方法对油菜秸秆的作用。具体而言，研究了野生型菌株（52J）和纤维二糖脱氢酶（CDH）缺陷型菌株（m4D）对秸秆的影响。与未处理的秸秆相比，用 52J 处理后的秸秆的木糖和葡萄糖含量显著降低，而用 m4D 处理后仅降低了木糖含量。与未处理的秸秆相比，真菌处理大大提高了木质素的可提取性。与未经处理的秸秆相比，m4D 处理的秸秆残渣的糖化导致葡萄糖产率的比例显著增加，这部分归因于 m4D 缺乏纤维素分解代谢。总的来说，这项研究的结果表明 CDH 有助于糙皮侧耳对纤维素的利用。此外，与未处理的生物质相比，用 m4D 处理木质纤维素材料在木质素提取率和糖化效率方面都有所提高，而不会因真菌分解代谢而损失基质。
