米糠固态发酵生产（）-2-（4-羟基苯氧基）丙酸
Production of ()-2-(4-hydroxyphenoxy) propionic acid by ZJB16007 in solid state fermentation using rice bran.
机构信息
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, China.
出版信息
Prep Biochem Biotechnol. 2020;50(8):781-787. doi: 10.1080/10826068.2020.1737939. Epub 2020 Mar 10.
-2-(4-hydroxyphenoxy)propionic acid (-HPPA) is a key chiral intermediate for phenoxypropionic acid herbicide synthesis. In this study, to improve the production of -HPPA with ZJB16007, the cultivation conditions in solid-state fermentation (SSF) were investigated. The effects of various substrates on -HPPA production were evaluated and the process parameters were also optimized. The results showed that rice bran was the optimal substrate for -HPPA production. The optimal medium components and cultivation conditions were: rice bran: silkworm chrysalis powder = 5.25: 2.25 (g: g), nutrient salts solution 12 mL which contained 50 g/L -PPA, pH 5.0, and cultivated at 28 °C for 11 days. Under the optimized conditions, the transformation of -HPPA was significantly improved and the yield of -HPPA reached 77.78%, which was 15.14% higher than that of the control (67.55%). Therefore, SSF may serve as an alternative for -HPPA production by ZJB16007.
2-(4-羟基苯氧基)丙酸（-HPPA）是苯氧丙酸类除草剂合成的关键手性中间体。本研究为提高 ZJB16007 生产 -HPPA 的能力，对其固态发酵（SSF）条件进行了研究。考察了不同底物对 -HPPA 生产的影响，并对工艺参数进行了优化。结果表明，米糠是生产 -HPPA 的最佳底物。最佳培养基组分为：米糠：蚕蛹粉=5.25:2.25（g:g），含 50g/L -PPA 的营养盐溶液 12mL，pH 值 5.0，28°C 培养 11 天。在优化条件下，-HPPA 的转化率显著提高，-HPPA 的产率达到 77.78%，比对照（67.55%）提高了 15.14%。因此，SSF 可能成为 ZJB16007 生产 -HPPA 的替代方法。
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