Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China.
Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin, China.
Anim Biotechnol. 2021 Oct;32(5):610-615. doi: 10.1080/10495398.2020.1736088. Epub 2020 Mar 10.
Myostatin () gene, also known as () gene, is a negative regulator of skeletal muscle growth and development, especially the number, size and type of muscle fibers. Its mutations contribute to the double-muscling (DBM) phenomenon which significantly increases the muscle mass. Hence, variations within / gene receive so much attention in several kinds of species such as bovines, poultries, goats, sheep, horses. A 5-base pairs (bp) indel in the 5' untranslated region (5'UTR) of goat / was verified to be significantly associated with growth traits except Inner Mongolia White Cashmere (IMWC) goats. Given that almost all sample sizes were below 150, we enlarged sample sizes to more than 500 to uncover the association between the 5-bp indel and growth traits in IMWC goats. Only two genotypes (deletion/deletion (DD) and insertion/deletion (ID)) were found, and DD genotypes were dominant genotypes. The detected locus displayed low genetic diversity (PIC = 0.090). Interestingly, the association analyses revealed that the 5-bp indel had a significant effect on the chest depth ( = 0.003), and DD genotypes were dominant genotypes. Hinted that the 5-bp indel could act as an effective marker in molecular marker-assisted selection (MAS) processes for selection of excellent goat individuals.
肌肉生长抑制素（）基因，也称为（）基因，是骨骼肌生长和发育的负调控因子，特别是肌肉纤维的数量、大小和类型。其突变导致双肌（DBM）现象，显著增加了肌肉质量。因此，/基因内的变异在牛、家禽、山羊、绵羊、马等多种物种中受到广泛关注。在山羊/的 5'非翻译区（5'UTR）中发现的 5 个碱基对（bp）缺失与生长性状显著相关，除了内蒙古白绒山羊（IMWC）。由于几乎所有样本量都低于 150，我们将样本量扩大到 500 多个，以揭示 IMWC 山羊中 5-bp 缺失与生长性状之间的关联。仅发现两种基因型（缺失/缺失（DD）和插入/缺失（ID）），且 DD 基因型为优势基因型。检测到的基因座显示出低遗传多样性（PIC = 0.090）。有趣的是，关联分析表明，5-bp 缺失对胸深（ = 0.003）有显著影响，且 DD 基因型为优势基因型。表明 5-bp 缺失可作为分子标记辅助选择（MAS）过程中选择优秀山羊个体的有效标记。
