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用微生物制剂接种牛粪可提高堆肥效率并促进其腐熟。

Inoculation of cattle manure with microbial agents increases efficiency and promotes maturity in composting.

作者信息

Li Jingbo, Wang Xitao, Cong Cong, Wan Leibing, Xu Yongping, Li Xiaoyu, Hou Fuqin, Wu Yanyan, Wang Lili

机构信息

1School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Hi-Tech Zone, Dalian, 116024 China.

Center for Food Safety of Animal Origin, Ministry of Education, Dalian, China.

出版信息

3 Biotech. 2020 Mar;10(3):128. doi: 10.1007/s13205-020-2127-4. Epub 2020 Feb 21.

Abstract

In this study, fresh cattle manure was mixed with rice straw at a ratio of 10:1 in fresh weight and then composted in a self-built, aerated static composting box, whose dimension was 1 m × 0.8 m × 0.8 m with a volume of approximately 0.6 m. To deal with the inconvenient and time-consuming problem of multiple stage inoculation, a single, one-time inoculation agent containing diverse microorganisms that are active at both the initial heating and thermophilic phases was developed. A total of 12 from 42 strains isolated from the none-inoculated Experiment 1 composting system were selected as microorganismal agents in Experiment 2 according to their species, prevalence and cultural temperature. 200 mL of each microorganism enrichment broth was mixed to the inoculation group at the beginning of composting. A total of 2400 mL of sterilize distilled water was added to the control group. The parameters of temperature, moisture, pH, C/N ratio, organic matter degradation, and germination index were investigated for both inoculation and control composting groups. Results showed that inoculation did not significantly shorten composting time. However, the pile temperature was increased with the maximum temperatures of 64.6 °C and 60.3 °C for the inoculation and control groups, respectively. The degradation of organic matter was accelerated ( < 0.05), and significantly higher GI value ( < 0.05) indicated that the maturity was promoted by the inoculation microorganism. This suggests that the final composting product would provide value as alternative source of nutrients for plants. Conclusively, we suggested a multiple microorganism inoculation method to increase the efficiency and promote maturity in cattle manure composting.

摘要

在本研究中,将新鲜牛粪与稻草按鲜重10:1的比例混合,然后在一个自行建造的通气静态堆肥箱中进行堆肥,该堆肥箱尺寸为1米×0.8米×0.8米,体积约为0.6立方米。为了解决多阶段接种不便且耗时的问题,开发了一种单一的一次性接种剂,其中含有在初始加热阶段和嗜热阶段均具有活性的多种微生物。根据从未接种的实验1堆肥系统中分离出的42株菌株的种类、流行程度和培养温度,在实验2中选择了12株作为微生物接种剂。在堆肥开始时,将200毫升每种微生物富集肉汤加入接种组。向对照组中加入总共2400毫升灭菌蒸馏水。对接种组和对照组堆肥的温度、湿度、pH值、碳氮比、有机物降解和发芽指数等参数进行了研究。结果表明,接种并没有显著缩短堆肥时间。然而,堆温有所升高,接种组和对照组的最高温度分别为64.6℃和60.3℃。有机物降解加快(P<0.05),且发芽指数显著更高(P<0.05),表明接种微生物促进了堆肥成熟。这表明最终的堆肥产品可作为植物营养的替代来源。总之,我们建议采用多种微生物接种方法来提高牛粪堆肥的效率并促进其成熟。

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