Deng Lin, Liang Haiyan, Han Yi
China-Japan Friendship Hospital, Beijing, China.
Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
Front Oncol. 2020 Feb 21;10:56. doi: 10.3389/fonc.2020.00056. eCollection 2020.
Explore the diagnostic and prognostic value of cyclooxygenase-2 and wnt3a/β-catenin pathway in endometrial cancer. A prospective cohort study of 93 women underwent hysterectomy at the China-Japan Friendship Hospital (61 patients with primary endometrial carcinoma, and 32 control patients with uterine prolapse or leiomyoma of uterus). Cox2 and β-catenin expression were determined by immunohistochemistry. The serum levels of cox2 and wnt3a were detected via ELISA assays. Patients with endometrial cancer showed overexpression of cox2 and β-catenin, as well as significantly higher serum levels of cox2 and wnt3a. The serum cox2 level, which is highly significant in predicting the risk of disease progression (RR, 9.617, 95% confidence interval, 1.162-79.622, = 0.036), showed good diagnostic and prognostic potential, with cut-off of 55 U/L, but alongside β-catenin expression in tissues, were related to poor prognosis (RR, 12.426; 95% confidence interval, 1.618-95.450; = 0.015). Serum levels of cox2 and wnt3a exhibited diagnostic value for endometrial cancer. Cox2 serum levels and β-catenin expression also showed potential value of prognostic prediction. Cox2 serum levels might be a potential marker for early diagnosis and prognosis prediction in endometrial cancer.
探讨环氧化酶-2和Wnt3a/β-连环蛋白通路在子宫内膜癌中的诊断和预后价值。一项前瞻性队列研究纳入了93名在中国-日本友好医院接受子宫切除术的女性（61例原发性子宫内膜癌患者，32例子宫脱垂或子宫肌瘤对照患者）。通过免疫组织化学测定Cox2和β-连环蛋白的表达。通过ELISA测定法检测血清中Cox2和Wnt3a的水平。子宫内膜癌患者表现出Cox2和β-连环蛋白的过表达，以及血清中Cox2和Wnt3a水平显著升高。血清Cox2水平在预测疾病进展风险方面具有高度显著性（RR，9.617，95%置信区间，1.162 - 79.622，P = 0.036），显示出良好的诊断和预后潜力，临界值为55 U/L，但与组织中β-连环蛋白的表达一起，与预后不良相关（RR，12.426；95%置信区间，1.618 - 95.450；P = 0.015）。血清Cox2和Wnt3a水平对子宫内膜癌具有诊断价值。Cox2血清水平和β-连环蛋白表达也显示出预后预测的潜在价值。Cox2血清水平可能是子宫内膜癌早期诊断和预后预测的潜在标志物。
