Morimoto Y, Ukida M, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
Gastroenterol Jpn. 1988 Oct;23(5):538-45. doi: 10.1007/BF02779486.
Fulminant hepatic failure (FHF) was produced in rats with intraperitoneal injection of D-galactosamine. 15NH4Cl (50 mg/kg of body weight) was injected into the rats via the tail vein. Arterial blood was drawn before and 5, 15, 30, 60 min after the injection of 15NH4Cl. 15N-ammonia, -urea, and -glutamine (amide and amino) were determined by gas chromatography and mass spectrometry. The plasma 15N-ammonia level was higher but decreased more rapidly in the FHF rats than in the control rats. This suggests that in FHF rats, the systemic vascular pool of ammonia is enlarged and ammonia clearance from blood is increased. The incorporation of 15N into urea was significantly different between the two groups. In FHF rats, the plasma urea-15N level rose 5 min after the injection, decreased at 15 min and was elevated again up to 60 min after injection. This biphasic change suggests that in FHF rats the incorporation of 15N into the extrahepatic glutamine pool is accelerated up to 15 min and that after 30 min a larger volume of glutamine-amide-15N is transferred to the urea cycle.
通过腹腔注射D-半乳糖胺在大鼠中诱导暴发性肝衰竭（FHF）。经尾静脉向大鼠注射15NH4Cl（50mg/kg体重）。在注射15NH4Cl之前以及注射后5、15、30、60分钟采集动脉血。通过气相色谱和质谱法测定15N-氨、-尿素和-谷氨酰胺（酰胺和氨基）。FHF大鼠的血浆15N-氨水平较高，但比对照大鼠下降得更快。这表明在FHF大鼠中，氨的全身血管池扩大，血液中氨的清除增加。两组之间15N掺入尿素的情况有显著差异。在FHF大鼠中，注射后5分钟血浆尿素-15N水平升高，15分钟时下降，注射后60分钟再次升高。这种双相变化表明，在FHF大鼠中，15N掺入肝外谷氨酰胺池的过程在15分钟内加速，30分钟后大量谷氨酰胺-酰胺-15N转移到尿素循环中。
