Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; Jilin Provincial Engineering Center of CWs Design in Cold Region & Beautiful Country Construction, Changchun 130102, China.
Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; Jilin Provincial Engineering Center of CWs Design in Cold Region & Beautiful Country Construction, Changchun 130102, China.
Environ Int. 2020 May;138:105628. doi: 10.1016/j.envint.2020.105628. Epub 2020 Mar 8.
The inhibition of salt stress on plant and microbial functions has led to the reduction of nitrogen removal capacity of constructed wetlands (CWs) under saline conditions. The mechanisms and effectiveness of bioaugmented CW (Bio-CW) microcosms with a salt-tolerant microbial inoculum were evaluated for nitrogen removal at different salinity levels. The results showed that the denitrification capacity of CWs was improved under saline conditions by adding the salt-tolerant microbial inoculum. At an EC of 15 mS/cm, the removal percentages of ammonia nitrogen (NH-N) and total nitrogen (TN) in Bio-CW microcosms (95.7% and 99.4%) on Day 5 were significantly (p < 0.05) higher than that in unbioaugmented CW (un-Bio-CW) microcosms (68.5% and 76.4%), respectively. The high throughput sequencing data of substrate samples indicated that the microbial community in the CWs was changed by the addition of the salt-tolerant microbial inoculum and the frequency of bacteria with nitrogen removal function was increased in the CWs. Furthermore, both growth and the TN accumulation capacity of plants in Bio-CW microcosms were promoted compared with the un-Bio-CW microcosms. In conclusion, the addition of the salt-tolerant microbial inoculum can enhance the nitrogen removal efficiency of CWs under saline condition via boosting the function of both microorganisms and plants.
盐胁迫抑制了植物和微生物的功能，导致盐度条件下人工湿地（CWs）的脱氮能力降低。本研究评估了添加耐盐微生物接种物的生物强化人工湿地（Bio-CW）微宇宙在不同盐度水平下的脱氮效果及其机制。结果表明，在盐度条件下添加耐盐微生物接种物可以提高 CWs 的反硝化能力。在 EC 值为 15 mS/cm 时，Bio-CW 微宇宙中氨氮（NH-N）和总氮（TN）的去除率（第 5 天分别为 95.7%和 99.4%）显著（p<0.05）高于未生物强化的 CW（un-Bio-CW）微宇宙（分别为 68.5%和 76.4%）。基质样品的高通量测序数据表明，耐盐微生物接种物的添加改变了 CWs 中的微生物群落，CWs 中具有脱氮功能的细菌出现的频率增加。此外，与 un-Bio-CW 微宇宙相比，Bio-CW 微宇宙中植物的生长和 TN 积累能力都得到了促进。总之，添加耐盐微生物接种物可以通过增强微生物和植物的功能来提高盐度条件下 CWs 的脱氮效率。
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