Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University in Kraków, Gronostajowa 2, 30-387, Kraków, Poland.
Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University in Kraków, Gronostajowa 2, 30-387, Kraków, Poland.
Forensic Sci Int. 2020 Apr;309:110230. doi: 10.1016/j.forsciint.2020.110230. Epub 2020 Feb 29.
In this study, the stability of the chemical composition of lipsticks after exposure to various factors (substrate, time, individual variability, the impact of smoking, the effect of consuming beverages) and storage conditions (laboratory, insolation, without access to light) was examined. The following three analytical methods were used in the study: Attenuated Total Reflection technique (ATR), Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Micellar Electrokinetic Capillary Chromatography (MEKC). Seven red lipsticks characterized by different chemical composition were analyzed. The analysis of variance (ANOVA) was used to estimate the impact of a given factor. It was noticed the lack of influence of individual variability, cigarette smoking and consumed beverages on the stability of the chemical composition of lipsticks. On the other hand, the changes in the chemical composition in lipstick traces depending on the time and storage conditions can be observed - especially when using the GC-MS method. In most cases, the results also indicated the possibility of identifying the lipstick left as a trace, using the ATR and MEKC method even after exposure to various factors and storage conditions. However, the main problem in the case of the ATR analysis is the occurrence of interference originating from the surface on which the trace of lipstick was applied. Ultimately, the conducted research provided evidence for the effectiveness of the MEKC method to the application in forensic science investigation.
本研究考察了口红在暴露于各种因素（基质、时间、个体差异、吸烟影响、饮料摄入的影响）和储存条件（实验室、光照、避光）下化学成分的稳定性。研究中使用了三种分析方法：衰减全反射技术（ATR）、气相色谱-质谱联用（GC-MS）和胶束电动毛细管色谱（MEKC）。分析了七种具有不同化学成分的红色口红。方差分析（ANOVA）用于估计给定因素的影响。研究发现，个体差异、吸烟和饮用饮料对口红化学成分的稳定性没有影响。另一方面，在时间和储存条件下，口红痕迹化学成分的变化是可以观察到的，特别是使用 GC-MS 方法时。在大多数情况下，即使在暴露于各种因素和储存条件后，ATR 和 MEKC 方法也有可能识别出留下的口红痕迹。然而，ATR 分析的主要问题是源自涂抹口红痕迹的表面的干扰的出现。最终，所进行的研究为 MEKC 方法在法庭科学调查中的应用提供了有效性证据。
