Titze Isabel, Krömker Volker
Department of Bioprocess Engineering and Microbiology, Hannover University of Applied Sciences and Arts, D-30453 Hannover, Germany.
University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary and Animal Sciences, Section for Production, Nutrition and Health, Gronnegardsvej 2, DK-1870 Frederiksberg C, Denmark.
Vet Sci. 2020 Mar 6;7(1):31. doi: 10.3390/vetsci7010031.
The antimicrobial activity of a phage mixture and a lactic acid bacterium against isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the germ density was determined [log cfu/mL]. The phage mixture consisted of the three obligatory lytic and -specific phages STA1.ST29, EB1.ST11 and EB1.ST27 (1:1:1). The selected strain with proven antimicrobial properties and the phage mixture were tested against in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.
研究了噬菌体混合物和乳酸菌对源自牛的分离株的抗菌活性，以期探讨其在治疗由细菌感染引起的乳房炎（乳腺炎）方面的潜在应用。用于接种的分离株取自乳腺炎病例的前乳区样本。为检测抗菌活性，测定了细菌密度的降低情况[log cfu/mL]。噬菌体混合物由三种 obligatory lytic 和特异性噬菌体 STA1.ST29、EB1.ST11 和 EB1.ST27（1:1:1）组成。将具有已证实抗菌特性的选定菌株和噬菌体混合物单独及联合用于牛奶中对相关菌株进行测试。在24小时的孵育期内，乳酸菌的应用仅显示出较低的杀菌能力。在37℃下24小时的孵育期内，噬菌体混合物及其与乳酸菌的组合对相关菌株均显示出高抗菌活性，其中只有噬菌体混合物显示出显著性。
