Dini C, Bolla P A, de Urraza P J
Center for Research and Development in Food Cryotechnology (CIDCA - CONICET-UNLP) CCT La Plata, La Plata, Argentina.
Cátedra de Microbiología, Facultad de Ciencias Exactas, UNLP, La Plata, Argentina.
J Appl Microbiol. 2016 Jul;121(1):78-88. doi: 10.1111/jam.13124. Epub 2016 May 30.
To assay the combination of phage and probiotics against EHEC in vitro on infected Hep-2 cells.
Phage and probiotics treatments on EHEC O157:H7-infected Hep-2 cells were assayed individually or combined. The effect of freeze-drying on phage and probiotic antimicrobial activity was also studied. While treatment with phage alone increased cell detachment caused by EHEC infection, the treatments with MM alone or in combination with phage proved to effectively diminish cell damage caused by EHEC infection. Combined treatment showed a decrease in apoptotic cell count of 57·3% and a reduction in EHEC adhesion to cell monolayer of 1·2 log CFU. The simultaneous use of phage and probiotics showed no antagonistic effect, and freeze-drying did not affect their antipathogenic activity.
The combination of phage and probiotics has great potential for reducing the number of pathogens adhered to epithelial cells during EHEC O157:H7 infection and attenuating the cytotoxic effect derived from it. Further in vivo assays are needed for assessing the actual effectiveness of the treatment.
This study presents a freeze-dried formulation of phage and probiotics capable of controlling EHEC infections and reducing epithelial cell damage in vitro.
在体外感染的Hep - 2细胞上检测噬菌体与益生菌联合对抗肠出血性大肠杆菌（EHEC）的效果。
分别或联合检测噬菌体和益生菌对EHEC O157:H7感染的Hep - 2细胞的处理效果。还研究了冷冻干燥对噬菌体和益生菌抗菌活性的影响。单独使用噬菌体处理会增加由EHEC感染引起的细胞脱落，而单独使用MM或与噬菌体联合使用的处理方法被证明能有效减轻EHEC感染引起的细胞损伤。联合处理使凋亡细胞计数减少了57.3%，EHEC对细胞单层的黏附减少了1.2 log CFU。同时使用噬菌体和益生菌未显示出拮抗作用，且冷冻干燥不影响它们的抗病原活性。
噬菌体与益生菌联合在减少EHEC O157:H7感染期间黏附于上皮细胞的病原体数量以及减轻由此产生的细胞毒性作用方面具有巨大潜力。需要进一步进行体内试验以评估该治疗方法的实际有效性。
本研究提出了一种能够在体外控制EHEC感染并减少上皮细胞损伤的噬菌体和益生菌冷冻干燥制剂。
