Fertility Center of Cha Gangnam Medical Center, Seoul 06135, Korea.
Department of Biomedical Science, CHA University, Gyeonggi-do 13488, Korea.
Genes (Basel). 2020 Mar 6;11(3):282. doi: 10.3390/genes11030282.
Baculoviral inhibitor of apoptosis repeat-containing 5 (), also known as survivin, is a member of the inhibitor of apoptosis (IAP) family of proteins and regulates the size of tissues through cell division control. The uterus is the most dynamically sized organ among tissues during the estrous cycle. Although is expressed in some terminally differentiated cells, the regulation of its expression in the uterus remains unknown. We investigated the regulation of expression in the mouse uterus. RT-PCR analysis showed that was expressed in various tissues, including the uterus; the expression level of was significantly higher at the diestrus stage. Immunohistochemistry and Western blotting analysis revealed that was more active in luminal and glandular epithelium than in endometrial stroma. In ovariectomized mice, expression in the uterus was gradually increased by estrogen treatment; however, progesterone injection decreased its expression. Estrogen-induced expression was blocked by treatment with estrogen receptor antagonist, ICI 182, 780 and progesterone-reduced expression was inhibited by the progesterone receptor antagonist RU486. These results suggest that expression is dynamically regulated by a combination of estrogen and progesterone via their receptor-mediated signaling.
杆状病毒 IAP 重复蛋白 5 ()，也被称为存活素，是凋亡抑制蛋白 (IAP) 家族的成员之一，通过细胞分裂控制调节组织的大小。在发情周期中，子宫是组织中体积变化最明显的器官。尽管 在一些终末分化的细胞中表达，但它在子宫中的表达调控尚不清楚。我们研究了 在小鼠子宫中的表达调控。RT-PCR 分析表明 在包括子宫在内的各种组织中表达；在动情期， 的表达水平显著升高。免疫组织化学和 Western blot 分析显示， 在腔上皮和腺上皮中的活性高于子宫内膜基质。在去卵巢小鼠中， 的表达在雌激素处理下逐渐增加；然而，孕激素注射降低了它的表达。雌激素诱导的 表达被雌激素受体拮抗剂 ICI 182,780 阻断，孕激素降低的 表达被孕激素受体拮抗剂 RU486 抑制。这些结果表明， 通过其受体介导的信号转导， 的表达受雌激素和孕激素的组合动态调节。
