Naef W, Munz K, Waser P G
Histochemistry. 1978 Dec 1;58(3):193-201. doi: 10.1007/BF00495719.
The influences of various fixatives on the vesicle size of the electric organ of Torpedo marmorate were investigated. Thin section and freeze etched preparations were examined under the electron microscope. In thin section increased vesicle diameters were observed compared with the freeze etched preparations. The same experiments in different torpedo fish led to significantly different vesicle sizes observed. Variations of the molarity, the pH and osmolarities result in particularly high differences in vesicle diameters. Using Karnovsky's method (1965) and a fixative consisting of 2.5% glutaraldehyde in 0.2 M cacodylate buffer, pH 7.2, results in vesicle sizes comparable to those reported by other authors. Results obtained from freeze etched preparations are not comparable in general with results from thin section experiments with the same fixative.
研究了各种固定剂对多斑电鳐电器官囊泡大小的影响。在电子显微镜下检查了超薄切片和冷冻蚀刻标本。与冷冻蚀刻标本相比，在超薄切片中观察到囊泡直径增加。在不同电鳐身上进行的相同实验导致观察到的囊泡大小存在显著差异。摩尔浓度、pH值和渗透压的变化导致囊泡直径的差异特别大。使用卡诺夫斯基方法（1965年）和由0.2M二甲胂酸钠缓冲液（pH 7.2）中2.5%戊二醛组成的固定剂，得到的囊泡大小与其他作者报道的相当。一般来说，冷冻蚀刻标本的结果与使用相同固定剂的超薄切片实验结果不可比。
