Kudavidanage Enoka P, Dissanayake D M I, Keerthirathna W L Rangi, Nishshanke N Lasni Wathima, Peiris L Dinithi C
Department of Natural Sciences, Sabaragamuwa University, Belhiloya 70140, Sri Lanka.
Department of Zoology, Faculty of Applied Sciences (Center for Biotechnology), University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka.
Biology (Basel). 2020 Mar 7;9(3):49. doi: 10.3390/biology9030049.
Pesticides are known to result in toxic insult. We aimed to evaluate Judo 40, the commercial formulation of chlorpyrifos on the neurological activities, fertility, and hormone levels of male rats. Male Wistar rats were treated orally with 1 mL of 20 or 50 mg/kg Judo 40. The doses were administered four times, twice a day. Sexual and exploratory behavior indices, fertility indices, serum androgen levels, blood acetylcholinesterase (BChE) levels, and neurological and muscular effects were evaluated. Serum testosterone and luteinizing hormone were significantly reduced in the rats receiving 50 mg/kg Judo 40. A reduction in viable implantation sites and live pups born were evident in the female rats mated with the male rats treated with the highest dose. Similarly, in the rats treated with the highest dose of Judo 40, a significant reduction in plasma BChE enzyme was observed. According to the results, prolonged Judo 40 exposure can cause impairment of the neurological alterations and sex hormones leading to impaired fertility. Therefore, chemical handlers should be educated on protection and risk minimization.
已知农药会造成毒性损害。我们旨在评估毒死蜱的商业制剂Judo 40对雄性大鼠神经活动、生育能力和激素水平的影响。将雄性Wistar大鼠口服给予1 mL 20或50 mg/kg的Judo 40。每天给药两次，共给药四次。评估了性行为和探索行为指标、生育指标、血清雄激素水平、血液乙酰胆碱酯酶（BChE）水平以及神经和肌肉效应。接受50 mg/kg Judo 40的大鼠血清睾酮和促黄体生成素显著降低。与接受最高剂量处理的雄性大鼠交配的雌性大鼠，其存活着床部位和出生的活仔数量明显减少。同样，在接受最高剂量Judo 40处理的大鼠中，观察到血浆BChE酶显著降低。根据结果，长期接触Judo 40会导致神经改变和性激素受损，从而导致生育能力受损。因此，应对化学品处理人员进行保护和风险最小化方面的教育。
