Comparative Medicine Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland;, Email:
Comparative Medicine Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland.
J Am Assoc Lab Anim Sci. 2020 May 1;59(3):310-321. doi: 10.30802/AALAS-JAALAS-19-000101. Epub 2020 Mar 10.
The goal of this study is to provide quantitative data on the ideal volume for intramuscular (IM) injections into the semimembranosus muscle of guinea pigs weighing between 320 to 410 grams. This evaluation comprised 2 experiments. The first was to assess dispersion leakage of intramuscularly injected iohexol, a radiocontrast agent commonly used in Computed Tomography (CT), based on analysis of in vivo imaging. The second used varying volumes of intramuscularly injected sodium chloride (0.9% NaCl) to assess pain and pathology associated with IM injection. Hartley guinea pigs were injected IM with varying volumes of either iohexol or sodium chloride (150, 300, 500, 1000 and 1500 μL). In the iohexol experiment, results suggest IM volumes of 150 and 300 μL remain within the target muscle. In the experiment using sodium chloride, pain and pathology did not increase as IM volume increased. The pathology noted was related to needle tract through the musculature rather than the volume size of the injectate. The results did not reveal a correlation between volume of IM 0.9% NaCl and pain levels. We conclude that volume size correlates more with precision and accuracy of delivery into the intended muscle tissue. Regarding tissue distribution, our findings also suggest that the optimal capacity for IM injection in the semimembranosus muscle should be less than 500 μL.
本研究旨在为 320 至 410 克重的豚鼠半膜肌肌内（IM）注射的理想容量提供定量数据。该评估包括两个实验。第一个实验是基于体内成像分析，评估 IM 注射的碘海醇（一种常用于计算机断层扫描（CT）的造影剂）的分散渗漏。第二个实验使用不同体积的 IM 注射氯化钠（0.9%NaCl）来评估与 IM 注射相关的疼痛和病理学。Hartley 豚鼠分别接受不同体积的碘海醇或氯化钠（150、300、500、1000 和 1500μL）的 IM 注射。在碘海醇实验中，结果表明 150 和 300μL 的 IM 体积仍在目标肌肉内。在使用氯化钠的实验中，随着 IM 体积的增加，疼痛和病理学并没有增加。所注意到的病理学与肌肉内的针道有关，而不是与注射物的体积大小有关。结果并未显示 IM 0.9%NaCl 体积与疼痛水平之间存在相关性。我们得出结论，体积大小与更精确和准确地将药物输送到预期的肌肉组织相关。关于组织分布，我们的研究结果还表明，半膜肌 IM 注射的最佳容量应小于 500μL。
