Collymore Chereen, Kent Laura, Ahn Sang Kyun, Xu Wenxi, Li Ming, Liu Jun, Turner Patricia V, Banks E Kate
Division of Comparative Medicine, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada;, Email:
Division of Comparative Medicine, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
Comp Med. 2018 Feb 1;68(1):41-47.
Guinea pigs are a commonly used model for tuberculosis vaccine research. Loss of body weight is the most frequently described humane endpoint for animals used in these studies. During a chronic study, we noted labored breathing in some tuberculosis-infected guinea pigs. To develop consistent humane endpoints for these guinea pigs, we performed an observational study using multiple clinical signs. A combination of body weight loss, labored breathing, and activity level during handling estimated the time to euthanasia within approximately 7 d. Histologic severity scores of lesions in the cranial or caudal lung lobe (or both) supported clinical endpoints. This study presents humane endpoints for the refinement of studies using guinea pigs in tuberculosis research.
豚鼠是结核病疫苗研究中常用的模型。体重减轻是这些研究中使用的动物最常描述的人道终点。在一项长期研究中，我们注意到一些感染结核病的豚鼠呼吸急促。为了为这些豚鼠制定一致的人道终点，我们使用多种临床症状进行了一项观察性研究。体重减轻、呼吸急促和处理过程中的活动水平相结合，可在大约7天内估计安乐死时间。颅侧或尾侧肺叶（或两者）病变的组织学严重程度评分支持临床终点。本研究提出了在结核病研究中使用豚鼠的研究优化的人道终点。
