Agroscope, Schwarzenburgstr, 161, 3003 Bern, Switzerland.
Agroscope, Tioleyre 4, 1725 Posieux, Switzerland.
Food Res Int. 2020 Apr;130:108996. doi: 10.1016/j.foodres.2020.108996. Epub 2020 Jan 11.
In vitro digestion systems are valuable tools for understanding and monitoring the complex behavior of food degradation during digestion, thus proving to be good candidates for replacing in vivo assays. The aim of the present work was to study protein hydrolysis in a selection of different protein sources using the harmonized INFOGEST static protocol: three isolated proteins (collagen, zein, and whey protein) and five foods (sorghum flour, wheat bran cereals, peanuts, black beans, and pigeon peas). The proteins of all the substrates were analyzed by SDS-PAGE and HPLC-MS/MS. Individual amino acid composition was analyzed by high-performance liquid chromatography (HPLC). EAA/NEAA (essential amino acids/ nonessential amino acids) ratios in the substrates from low to high were as follows: wheat bran cereals, peanuts, collagen, zein, whey protein, sorghum, pigeon peas, and black beans. The results revealed sorghum, whey protein, and zein as good sources of BCAA. In all substrates, no intact protein from the substrates was visually detected by SDS-PAGE after the intestinal phase of in vitro digestion with the INFOGEST protocol. However, digestion-resistant peptides were detected in all substrates after the intestinal digestion phase. Protein hydrolysis was high in whey protein isolate and pigeon pea and low for wheat bran cereals and bovine collagen.
体外消化系统是理解和监测食物在消化过程中复杂降解行为的有用工具，因此被证明是替代体内测定的良好候选物。本研究旨在使用协调的 INFOGEST 静态方案研究不同蛋白质来源的蛋白质水解：三种分离蛋白（胶原蛋白、玉米醇溶蛋白和乳清蛋白）和五种食物（高粱粉、小麦麸皮谷物、花生、黑豆和兵豆）。所有底物的蛋白质均通过 SDS-PAGE 和 HPLC-MS/MS 进行分析。通过高效液相色谱法（HPLC）分析个别氨基酸组成。底物中的 EAA/NEAA（必需氨基酸/非必需氨基酸）比值从低到高依次为：小麦麸皮谷物、花生、胶原蛋白、玉米醇溶蛋白、乳清蛋白、高粱、兵豆和黑豆。结果表明，高粱、乳清蛋白和玉米醇溶蛋白是支链氨基酸的良好来源。在用 INFOGEST 方案进行体外消化的肠内阶段后，通过 SDS-PAGE 无法在所有底物中观察到来自底物的完整蛋白质。然而，在肠内消化阶段后，所有底物中均检测到消化抗性肽。乳清蛋白分离物和兵豆的蛋白质水解度较高，而小麦麸皮谷物和牛胶原蛋白的蛋白质水解度较低。
