Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, China.
Vet Parasitol. 2021 Sep;297:109069. doi: 10.1016/j.vetpar.2020.109069. Epub 2020 Mar 5.
Trichinellosis caused by Trichinella spiralis (T. spiralis) is an important public health problem. DNase II is an acidic endonuclease that catalyzes the degradation of DNA into oligonucleotides. DNase II-7 has been detected at the adult stage of T. spiralis and has been examined in excretory/secretory products. Previous studies have indicated that the DNase II-7 recombinant protein has a high rate of protection against T. spiralis infection in mice. In the present study, the protective effect of DNase II-7 recombinant protein against T. spiralis infection in Large White pigs was further explored. The humoral and cellular immune responses to the DNase II-7 recombinant protein were evaluated, including the dynamic trends of specific IgG, IgG1, IgG2a and IgM antibodies levels, as well as the levels of Th1 (IFN-γ and IL-2) and Th2 (IL-10 and IL-4) cytokines in serum. Our results showed that a Th1 dominated Th1/Th2 mixed immune response was induced by the DNase II-7 recombinant protein for all the time or a short period after vaccination. And the DNase II-7 recombinant protein induced partial protection against T. spiralis infection in pigs, compared to the control group. Our results showed that the DNase II-7 recombinant protein group displayed a 45.7 % reduction in the muscle larvae burden five weeks after being challenged. This study suggested that DNaseII-7 recombinant protein could be used as a potential candidate vaccine against T. spiralis infection in pigs.
旋毛虫病是由旋毛形线虫（T. spiralis）引起的，是一个重要的公共卫生问题。DNase II 是一种酸性内切核酸酶，能够催化 DNA 降解为寡核苷酸。DNase II-7 已在旋毛虫的成虫阶段被检测到，并在排泄/分泌产物中进行了研究。先前的研究表明，DNase II-7 重组蛋白在小鼠中对旋毛虫感染具有很高的保护率。在本研究中，进一步探讨了 DNase II-7 重组蛋白对大白猪旋毛虫感染的保护作用。评估了对 DNase II-7 重组蛋白的体液和细胞免疫反应，包括特异性 IgG、IgG1、IgG2a 和 IgM 抗体水平的动态趋势，以及血清中 Th1（IFN-γ 和 IL-2）和 Th2（IL-10 和 IL-4）细胞因子的水平。我们的结果表明，DNase II-7 重组蛋白诱导了 Th1 主导的 Th1/Th2 混合免疫反应，持续或接种后短期。并且，与对照组相比，DNase II-7 重组蛋白对猪旋毛虫感染具有部分保护作用。我们的结果表明，在 challenged 后五周，DNase II-7 重组蛋白组的肌肉幼虫负荷减少了 45.7%。本研究表明，DNaseII-7 重组蛋白可用作抗猪旋毛虫感染的潜在候选疫苗。
