Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
Department of Human Parasitology, School of Basic Medicine, Hubei University of Medicine, Shiyan, China.
PLoS Negl Trop Dis. 2021 May 10;15(5):e0009408. doi: 10.1371/journal.pntd.0009408. eCollection 2021 May.
Trichinellosis is a major foodborne parasitosis caused by Trichinella spiralis. In the present study, a serine protease gene from an adult T. spiralis (Ts-Adsp) cDNA library was cloned, expressed in Escherichia coli and purified by Ni-affinity chromatography. Previous studies of our laboratory have found that mice vaccinated with recombinant Ts-Adsp protein (rTs-Adsp) exhibited partial protection against T. spiralis infection. In this study, the protective effect of rTs-Adsp against T. spiralis infection in pigs was further explored. The cell-mediated and humoral immune responses induced by rTs-Adsp were measured, including the dynamic trends of specific antibody levels (IgG, IgG1, IgG2a and IgM), as well as the levels of cytokines (IFN-γ, IL-2, IL-4, and IL-10) in the serum. Moreover, the changes in T lymphocytes, B lymphocytes, and neutrophils were measured to evaluate cellular immune responses in pigs vaccinated with rTs-Adsp. The results indicated that a Th1-Th2 mixed immune response with Th1 predominant was induced by rTs-Adsp after vaccination. Flow cytometric analysis showed that the proportions of CD4+ T cells, B cells, and neutrophils in the immunized groups were significantly increased. Furthermore, pigs vaccinated with rTs-Adsp exhibited a 50.9% reduction in the muscle larvae burden, compare with pigs from the PBS group five weeks after challenged. Our results suggested that rTs-Adsp elicited partial protection and it could be a potential target molecule for preventing and controlling Trichinella transmission from pigs to human.
旋毛虫病是一种由旋毛虫引起的重要食源性寄生虫病。本研究从旋毛虫成虫 cDNA 文库中克隆了一个丝氨酸蛋白酶基因，并在大肠杆菌中表达，然后通过 Ni-亲和层析进行纯化。本实验室的先前研究发现，用重组 Ts-Adsp 蛋白（rTs-Adsp）免疫的小鼠对旋毛虫感染具有部分保护作用。在本研究中，进一步探索了 rTs-Adsp 对猪旋毛虫感染的保护作用。测量了 rTs-Adsp 诱导的细胞介导和体液免疫反应，包括特异性抗体水平（IgG、IgG1、IgG2a 和 IgM）的动态趋势，以及血清中细胞因子（IFN-γ、IL-2、IL-4 和 IL-10）的水平。此外，还测量了 T 淋巴细胞、B 淋巴细胞和中性粒细胞的变化，以评估接种 rTs-Adsp 的猪的细胞免疫反应。结果表明，rTs-Adsp 接种后诱导了 Th1-Th2 混合免疫反应，以 Th1 为主。流式细胞术分析表明，免疫组中 CD4+T 细胞、B 细胞和中性粒细胞的比例显著增加。此外，与 PBS 组相比，接种 rTs-Adsp 的猪在攻毒后五周肌肉幼虫负荷减少了 50.9%。我们的结果表明，rTs-Adsp 引起了部分保护作用，它可能是预防和控制从猪到人传播旋毛虫的潜在靶分子。
