Azm Center for Research in Biotechnology and its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon; Institute of Chemistry and Processes for Energy, Environment and Health ICPEES UMR 7515 Group of Physical Chemistry of the Atmosphere, University of Strasbourg, Strasbourg, France; Lebanese American University, School of Engineering, Byblos, Lebanon.
Institute of Chemistry and Processes for Energy, Environment and Health ICPEES UMR 7515 Group of Physical Chemistry of the Atmosphere, University of Strasbourg, Strasbourg, France.
J Chromatogr A. 2020 Jun 21;1621:461006. doi: 10.1016/j.chroma.2020.461006. Epub 2020 Mar 3.
This paper reports an optimized multiresidue extraction strategy based on the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) extraction procedure and on solid-phase microextraction (SPME) for the simultaneous screening of 120 pesticides, 16 polycyclic aromatic hydrocarbons, and 22 polychlorinated biphenyls from the terrestrial snail Helix aspersa. The optimized extraction method was based on QuEChERS using acetonitrile, followed by dispersive-Solid-phase extraction clean-up using primary secondary amine and octadecyl (C18) sorbents. The obtained extracts were analyzed by liquid chromatography coupled with tandem mass spectrometry and gas chromatography coupled with tandem mass spectrometry. This latest technique was preceded by a pre-concentration step using SPME with appropriate fibers. Afterwards, the method was validated for its linearity, sensitivity, recovery, and precision. Results showed high sensitivity, accuracy, and precision, with limits of detection and quantification lower than 20 ng g for most considered pollutants. Both inter and intra-day analyses revealed low relative standard deviation (%), which was lower than 20% for most targeted compounds. Moreover, the obtained regression coefficient (R) was higher than 0.98 and the recoveries were higher than 60% for the majority of the assessed pollutants.
本文报道了一种优化的多残留提取策略，基于 QuEChERS 提取程序和固相微萃取（SPME），从陆生蜗牛 Helix aspersa 中同时筛选 120 种农药、16 种多环芳烃和 22 种多氯联苯。优化的提取方法基于 QuEChERS 用乙腈，然后用初级二次胺和十八烷基（C18）吸附剂进行分散固相萃取净化。所得提取物通过液相色谱-串联质谱和气相色谱-串联质谱进行分析。在使用适当纤维的 SPME 进行预浓缩步骤之前，采用了这种最新技术。然后，对该方法的线性、灵敏度、回收率和精密度进行了验证。结果表明，该方法具有较高的灵敏度、准确性和精密度，大多数考虑的污染物的检测限和定量限均低于 20ng g-1。日内和日间分析的相对标准偏差（%）均较低，大多数目标化合物的相对标准偏差均低于 20%。此外，对于大多数评估的污染物，获得的回归系数（R）高于 0.98，回收率高于 60%。
