Centre d'expertise en analyse environnementale du Québec, ministère du Développement durable, de l'Environnement et de la Lutte contre les changements climatiques, Québec, Canada; Institut national de la recherche scientifique - Institut Armand-Frappier, Laval, Canada.
Centre d'expertise en analyse environnementale du Québec, ministère du Développement durable, de l'Environnement et de la Lutte contre les changements climatiques, Québec, Canada.
Talanta. 2017 Apr 1;165:332-338. doi: 10.1016/j.talanta.2016.12.080. Epub 2016 Dec 27.
In this study, a fast and rugged method is presented for the analysis of PCBs, PAHs, PBDEs and PCDD/Fs in biological tissues using a simple Quick, Easy, Cheap, Efficient, Rugged and Safe (QuEChERS) extraction and a clean-up by Gel Permeation Chromatography (GPC) and silica Solid Phase Extraction (SPE). Development was performed on blue mussels (Mytilus edulis) and Atlantic salmon (Salmo salar) for evaluation of two ranges of lipid and water content of biological tissues. Statistical validation was performed with Atlantic salmon samples. Forty-five PAHs were analyzed including the priority list of the US EPA and the European Union with 41 PCBs, 24 PBDEs and 17 PCDD/Fs. Instrumental analyses were performed on Gas Chromatography - High Resolution Mass Spectrometry (GC-HRMS). Accuracy was evaluated for PCBs and PCDD/Fs with a certified reference material furnished by the National Research Council Canada (NRCC) and also compared with results obtained by the conventional Soxhlet extraction. Statistical validation showed recoveries for PCBs, PAHs, PBDEs and PCDD/Fs close to 100% with average Relative Standard Deviation (RSD) lower than 10% and internal standard recoveries in the range of 70% with average RSD ranging from 5-15%. Average calculated Method Detection Limits (MDLs) were lower than 0.05μg/Kg for PCBs, 0.2μg/Kg for PAHs and PBDEs and 1ng/Kg for PCDD/Fs. The method is a faster and cheaper alternative to the time-consuming conventional method that has been used in most environmental laboratories.
本研究提出了一种快速而稳健的方法，用于使用简单的快速、简便、廉价、有效、坚固和安全（QuEChERS）提取和凝胶渗透色谱（GPC）和硅胶固相萃取（SPE）进行净化，分析生物组织中的多氯联苯（PCBs）、多环芳烃（PAHs）、多溴二苯醚（PBDEs）和二恶英/呋喃（PCDD/Fs）。该方法在贻贝（Mytilus edulis）和大西洋鲑鱼（Salmo salar）中进行了开发，以评估两种生物组织的脂质和水分含量范围。采用大西洋鲑鱼样品进行了统计学验证。分析了包括美国环保局和欧盟优先名单在内的 45 种多环芳烃，以及 41 种多氯联苯、24 种多溴二苯醚和 17 种二恶英/呋喃。仪器分析采用气相色谱-高分辨率质谱（GC-HRMS）进行。采用加拿大国家研究委员会（NRCC）提供的认证参考物质对多氯联苯和二恶英/呋喃进行了准确性评估，同时还与传统索氏提取的结果进行了比较。统计验证表明，多氯联苯、多环芳烃、多溴二苯醚和二恶英/呋喃的回收率接近 100%，平均相对标准偏差（RSD）低于 10%，内标回收率在 70%范围内，平均 RSD 为 5-15%。平均计算方法检测限（MDLs）低于 0.05μg/Kg 的多氯联苯、0.2μg/Kg 的多环芳烃和 PBDEs 以及 1ng/Kg 的二恶英/呋喃。与大多数环境实验室中使用的耗时的传统方法相比，该方法是一种更快、更便宜的替代方法。
