Department of Toxicology, School of Public Heath, Shenyang Medical College, Shenyang, 110034, Liaoning Province, People's Republic of China.
Department of Environmental Engineering and Bioengineering, Shenyang Institute of Science and Technology, Shenyang, 110167, Liaoning Province, People's Republic of China.
Environ Sci Pollut Res Int. 2020 May;27(14):17290-17302. doi: 10.1007/s11356-020-08156-x. Epub 2020 Mar 10.
The effect of prenatal bisphenol A (BPA) exposure is increasingly concerned. We investigated the effect of maternal BPA exposure during pregnancy on male offspring and its potential mechanism. Thirty pregnant Sprague Dawley (SD) rats were randomly divided into exposed and control groups. At PND56, the number of sperm, luteinizing hormone, and testosterone in the BPA-exposed group decreased, and testicular tissue structure was damaged in offsprings. At GD20, the miRNA profile in the testis of male offspring was examined and the expression levels of 28 deregulated miRNAs were validated by qRT-PCR. We found that miR-361-5p, miR-203a-3p, and miR-19b-2-5p had significantly different expression levels in the testis. These results suggest that maternal exposure to BPA can lead to differential changes in progeny miRNAs, which will provide direction for future in-depth mechanisms of reproductive injury.
孕期双酚 A（BPA）暴露的影响越来越受到关注。我们研究了母体孕期暴露于 BPA 对雄性后代的影响及其潜在机制。30 只怀孕的 Sprague Dawley（SD）大鼠被随机分为暴露组和对照组。在 PND56 时，BPA 暴露组雄性后代的精子数量、黄体生成素和睾酮减少，睾丸组织结构受损。在 GD20 时，检测了雄性后代睾丸中的 miRNA 图谱，并通过 qRT-PCR 验证了 28 个失调 miRNA 的表达水平。我们发现 miR-361-5p、miR-203a-3p 和 miR-19b-2-5p 在睾丸中的表达水平有明显差异。这些结果表明，母体暴露于 BPA 可导致后代 miRNA 的差异变化，这将为生殖损伤的深入机制研究提供方向。
