Section for Integrative Physiology, The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
Section for Integrative Physiology, Department of Molecular Medicine & Surgery, Karolinska Institutet, Stockholm, Sweden.
Epigenomics. 2020 Apr;12(8):701-713. doi: 10.2217/epi-2019-0391. Epub 2020 Mar 11.
Innate circadian rhythms are critical for optimal tissue-specific functions, including skeletal muscle, a major insulin-sensitive tissue responsible for glucose homeostasis. We determined whether transcriptional oscillations are associated with CpG methylation changes in skeletal muscle. We performed rhythmicity analysis on the transcriptome and CpG methylome of circadian synchronized myotubes. We identified several transcripts and CpG-sites displaying oscillatory behavior, which were enriched with Gene Ontology terms related to metabolism and development. Oscillating CpG methylation was associated with rhythmic expression of 31 transcripts. Although circadian oscillations may be regulated by rhythmic DNA methylation, strong rhythmic associations between transcriptome and CpG methylation were not identified. This resource constitutes a transcriptomic/epigenomic atlas of skeletal muscle and regulation of circadian rhythms.
内源性昼夜节律对于组织特异性功能（包括骨骼肌）至关重要，骨骼肌是主要的胰岛素敏感组织，负责葡萄糖稳态。我们确定了转录振荡是否与骨骼肌中的 CpG 甲基化变化相关。我们对生物钟同步的肌管的转录组和 CpG 甲基组进行了节律性分析。我们发现了几个显示振荡行为的转录本和 CpG 位点，这些基因富集了与代谢和发育相关的基因本体论术语。振荡的 CpG 甲基化与 31 个转录本的节律性表达相关。虽然昼夜节律可能受节律性 DNA 甲基化调控，但并未发现转录组和 CpG 甲基化之间存在强烈的节律关联。本资源构成了骨骼肌转录组/表观基因组图谱和昼夜节律调节。
