Shen Jianshuang, Wu Yutong, Jiang Zhiyi, Xu Yang, Zheng Tangchun, Wang Jia, Cheng Tangren, Zhang Qixiang, Pan Huitang
Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, Beijing, China.
National Engineering Research Center for Floriculture, Beijing, China.
Physiol Mol Biol Plants. 2020 Jan;26(1):173-188. doi: 10.1007/s12298-019-00731-y. Epub 2019 Dec 9.
The qRT-PCR method has been widely used to detect gene expression level in plants, helping to understand the molecular mechanisms. However, there are few researches which focus on the selection of the internal reference genes in . To select the appropriate reference genes of aimed at qRT-PCR normalization, twelve candidate reference genes were selected from our transcriptome data. Their expression was assessed by RT-PCR analysis in 47 samples, including 12 species cultivars, different organs and tissues. GeNorm, NormFinder, and BestKeeper software were used to select the appropriate reference genes, and were used to verify the accuracy of the outcome. The results showed that was a stable reference gene in leaves of twelve germplasms and in different developmental stages of fruits. , + , and + + were stable reference genes in different organs. and were stable reference genes in different flower tissues and different developmental stages of the flower buds. When plants were stressed with PEG or ABA, + + was the stable reference gene group for qRT-PCR. The results provided the basis for investigating the physiological and biochemical processes of related to medicinal and ornamental properties, and drought-resistance in the level of gene expression.
qRT-PCR方法已被广泛用于检测植物中的基因表达水平，有助于了解分子机制。然而，很少有研究关注[具体植物名称]中内参基因的选择。为了选择适用于qRT-PCR标准化的[具体植物名称]参考基因，从我们的转录组数据中选择了12个候选参考基因。通过RT-PCR分析评估了它们在47个[具体植物名称]样本中的表达，这些样本包括12个品种、不同器官和组织。使用GeNorm、NormFinder和BestKeeper软件选择合适的参考基因，并使用[具体方法名称]验证结果的准确性。结果表明，[基因名称1]是12种[具体植物名称]种质叶片和果实不同发育阶段的稳定参考基因。[基因名称2]、[基因名称3]和[基因名称4]是不同器官中的稳定参考基因。[基因名称5]和[基因名称6]是不同花组织和花芽不同发育阶段的稳定参考基因。当[具体植物名称]植株受到PEG或ABA胁迫时，[基因名称7]是qRT-PCR的稳定参考基因组。这些结果为在基因表达水平上研究[具体植物名称]与药用和观赏特性以及抗旱性相关的生理生化过程提供了依据。
