Ghariani Salma, Charfeddine Amina, Amari Marwen, Chakroun Mohamed, Neila Trifi-Farah
1Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.
2Laboratoire des Productions Animales et Fourragères, Institut National de Recherche Agronomique de la Tunisie, Université de Carthage, Rue Hédi Karray, 2049 Ariana, Tunisia.
Physiol Mol Biol Plants. 2020 Feb;26(2):331-339. doi: 10.1007/s12298-019-00749-2. Epub 2020 Jan 20.
To establish phylogenetic relationships and estimate the intra and interspecific divergence, the amplification and the sequencing of the internal transcribed spacers of ribosomal DNA (ITS = ITS1 + 5.8S + ITS2) were analyzed in Tunisian complex - DNA. These spacer regions have evolved mainly by point mutations. Results revealed a high level of polymorphism within studied species. Significant similarities were observed between these two species and showed the existence of an important phylogenetic relationship. Besides, this molecular approach has revealed two new clusterings, with a homologous ITS gene namely: and subsp. This could be explained by the conservation of an ancestral ITS gene in some fescue plants. Thus, Tunisian tall fescue and perennial ryegrass may derive from and subsp. Considerable morphology and bioclimatic distribution similarities were discovered in ITS sequences within the same species. This study can be of great help to identify suitable accessions that could be used in local fescue and ryegrass improvement program.
为了建立系统发育关系并估计种内和种间差异，对突尼斯复合DNA中核糖体DNA的内部转录间隔区（ITS = ITS1 + 5.8S + ITS2）进行了扩增和测序分析。这些间隔区主要通过点突变进化。结果显示在所研究的物种中存在高水平的多态性。在这两个物种之间观察到显著的相似性，并表明存在重要的系统发育关系。此外，这种分子方法揭示了两个新的聚类，具有同源的ITS基因，即： 和 亚种。这可以通过某些羊茅属植物中祖先ITS基因的保守性来解释。因此，突尼斯高羊茅和多年生黑麦草可能源自 和 亚种。在同一物种的ITS序列中发现了相当大的形态和生物气候分布相似性。这项研究对于识别可用于当地羊茅和黑麦草改良计划的合适种质可能有很大帮助。
