Lestari Dela Ayu, Oikawa Takuro, Sutopo Sutopo, Purbowati Endang, Setiaji Asep, Kurnianto Edy
Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang, Central Java 50275, Indonesia.
Department of Subtropical Agro-Environmental Sciences, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
Vet World. 2020 Jan;13(1):127-133. doi: 10.14202/vetworld.2020.127-133. Epub 2020 Jan 18.
This study aimed to identify the effect of the insulin-like growth factor 1 () gene on growth, to uncover the genetic marker at the gene, and to predict growth performance by analyzing growth models of Kejobong goats based on their genotype.
DNA and records of body weight (BW) and body measurements (BM) of Kejobong goats were collected, the gene was amplified from the DNA template by polymerase chain reaction (PCR); the PCR products were then sequenced to determine single nucleotide polymorphisms (SNP). Linear mixed model (LMM) was used to analyze the association between SNP and growth traits. Four non-linear growth models were analyzed using non-LMM to describe the growth model and to compare the growth within genotypes.
An SNP at intron 4 (g5752G→C) genotyped into GG and CC was significantly associated with BW and BM. Goats of genotype GG had a significantly higher BW and BM (p<0.05) than those of genotype CC. Growth analysis showed that the von Bertalanffy model was the most fit for describing BW, the Brody model for chest width and hip height, the Gompertz and Logistic models for heart girth, and the von Bertalanffy and Gompertz models for hip width.
An SNP at intron 4 of the gene was associated with the growth trait and was usable as a genetic marker candidate for improvement of growth traits of Kejobong goats while von Bertalanffy model provides proper and accurate estimates of parameters to describe the growth performance of Kejobong goats.
本研究旨在确定胰岛素样生长因子1（）基因对生长的影响，揭示该基因的遗传标记，并通过分析基于基因型的科约邦山羊生长模型来预测其生长性能。
收集科约邦山羊的DNA以及体重（BW）和体尺（BM）记录，通过聚合酶链反应（PCR）从DNA模板中扩增该基因；然后对PCR产物进行测序以确定单核苷酸多态性（SNP）。使用线性混合模型（LMM）分析SNP与生长性状之间的关联。使用非LMM分析四种非线性生长模型，以描述生长模型并比较基因型内的生长情况。
内含子4处的一个SNP（g5752G→C），基因型分为GG和CC，与BW和BM显著相关。GG基因型的山羊BW和BM显著高于CC基因型的山羊（p<0.05）。生长分析表明，冯·贝塔朗菲模型最适合描述BW，布罗迪模型适合描述胸围和臀高，冈珀茨和逻辑斯蒂模型适合描述心围，冯·贝塔朗菲和冈珀茨模型适合描述臀宽。
该基因内含子4处的一个SNP与生长性状相关，可作为改善科约邦山羊生长性状的候选遗传标记，而冯·贝塔朗菲模型能提供恰当且准确的参数估计来描述科约邦山羊的生长性能。
