Jamali Samar, Tavakoly Talayeh, Mojtahedi Ali, Shenagari Mohammad
Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran.
Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Infect Drug Resist. 2020 Feb 25;13:651-657. doi: 10.2147/IDR.S230335. eCollection 2020.
and as an important part of family are important causes of both community- and hospital-acquired infections. The present study aimed to investigate the prevalence of antibiotics resistance and molecular characteristics of uropathogenic isolates of and in Iranian patients.
This cross-sectional study performed on 223 and 68 isolates obtained from hospitalized patients in the north of Iran. The isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Disk diffusion method was applied to determine antibiotic susceptibility pattern. The presence of β-lactamases encoding genes was evaluated by PCR method. Analysis of the mutations and homology among sequences was done by the CLC sequence viewer (Qiagen, Denmark), and phylogenetic trees were constructed by the neighbor-joining method (Bootstrap: 1000 times).
The overall rates of extended-spectrum β-lactamases (ESBLs)-producing and isolates were 37.7% and 32.4%, respectively. The overall presence of , , and genes was detected in 16 (5.5%), 12 (4.1%), and 48 (16.4%) of isolates, respectively. The neighbor-joining analysis for KU985246.1 strain showed that the most related sequences were from China, Singapore, UK, Thailand, and Bangladesh. While KU985245.1 strains were mostly related to sequences form Myanmar, and China.
In summary, the remarkable rate of ESBL-producing uropathogenic along with the first prevalence of NDM-1 β-lactamases can be a serious concern in our region.
作为家庭的重要组成部分，[具体细菌名称未给出]是社区获得性感染和医院获得性感染的重要原因。本研究旨在调查伊朗患者中[具体细菌名称未给出]尿路致病性分离株的抗生素耐药性流行情况及分子特征。
本横断面研究对从伊朗北部住院患者中分离出的223株[具体细菌名称未给出]和68株[具体细菌名称未给出]进行。通过标准微生物学试验鉴定分离株，并通过API 20E试纸条进行确认。采用纸片扩散法确定抗生素敏感性模式。通过PCR方法评估β-内酰胺酶编码基因的存在情况。利用CLC序列查看器（丹麦Qiagen公司）对序列中的突变和同源性进行分析，并采用邻接法构建系统发育树（自展值：1000次）。
产超广谱β-内酰胺酶（ESBLs）的[具体细菌名称未给出]和[具体细菌名称未给出]分离株的总体发生率分别为37.7%和32.4%。分别在16株（5.5%）、12株（4.1%）和48株（16.4%）分离株中检测到[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因的总体存在情况。对[具体细菌名称未给出]KU985246.1菌株的邻接分析表明，最相关的[具体细菌名称未给出]序列来自中国、新加坡、英国、泰国和孟加拉国。而[具体细菌名称未给出]KU985245.1菌株大多与来自缅甸和中国的[具体细菌名称未给出]序列相关。
总之，产ESBLs的尿路致病性[具体细菌名称未给出]的高发生率以及NDM-1β-内酰胺酶的首次流行可能是我们地区的一个严重问题。
